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首页> 外文期刊>Bioprocess and Biosystems Engineering >Comparative evaluation of phototrophic microtiter plate cultivation against laboratory-scale photobioreactors
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Comparative evaluation of phototrophic microtiter plate cultivation against laboratory-scale photobioreactors

机译：相对于实验室规模的光生物反应器的光养微量滴定板培养的比较评价

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摘要

Extended cultivation times, rendering phototrophic bioprocess development time inefficient, resulted in the recent development of micro-photobioreactors enabling accelerated process development. However, especially for laboratory photobioreactors, only little is known concerning the influence of design on process performance. Thus, the aim of the present investigation was to evaluate the scalability of a microtiter plate-based parallelized micro-photobioreactor against a representative set of established laboratory photobioreactors. Lipid production by Chlorella vulgaris was used as a model system. During exponential growth, the microtiter plate cultures achieved maximal growth rates of ca. 1.44 +/- 0.02 day(-1) being in good agreement with the larger systems. Moreover, cultures in the micro-photobioreactor could be kept in the exponential phase up to the highest biomass concentrations most probably due to the beneficial light supply at this scale. Compared to the shake flask and test tube cultures, microtiter plate cultivation achieved an equivalent biomass yield, lipid content, and lipid fingerprint. In contrast, the flat-panel process resulted only in marginal productivity due to insufficient light supply. Thus, microtiter plates showed good scalability to the investigated laboratory photobioreactors as overall differences were rather small taking the differing scales into account.
机译：延长的培养时间，使光养生物过程的开发时间效率低下，导致了最近开发的微型光生物反应器能够加速过程的发展。但是，特别是对于实验室光生物反应器，关于设计对工艺性能的影响知之甚少。因此，本研究的目的是评估基于微量滴定板的平行化微型光生物反应器对已建立的代表性实验室光生物反应器的可扩展性。小球藻产生的脂质被用作模型系统。在指数生长过程中，微量滴定板培养物达到了约1000的最大生长速率。 1.44 +/- 0.02天（-1）与大型系统非常吻合。此外，微光生物反应器中的培养物可以保持指数级直至最高生物质浓度，这很可能是由于在这种规模下的有益光供应。与摇瓶和试管培养相比，微量滴定板培养获得了相当的生物量产量，脂质含量和脂质指纹。相反，由于光供应不足，平板工艺仅导致边际生产率。因此，微量滴定板对所研究的实验室光生物反应器显示出良好的可扩展性，因为考虑到不同的规模，总体差异很小。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2017年第5期|663-673|共11页
  • 作者

    Morschett Holger; Schiprowski Danny; Rohde Jannis; Wiechert Wolfgang; Oldiges Marco;

  • 作者单位

    Forschungszentrum Julich, Inst Bio & Geosci, IBG Biotechnol 1, Wilhelm Johnen Str, D-52428 Julich, Germany;

    Forschungszentrum Julich, Inst Bio & Geosci, IBG Biotechnol 1, Wilhelm Johnen Str, D-52428 Julich, Germany;

    Forschungszentrum Julich, Inst Bio & Geosci, IBG Biotechnol 1, Wilhelm Johnen Str, D-52428 Julich, Germany;

    Forschungszentrum Julich, Inst Bio & Geosci, IBG Biotechnol 1, Wilhelm Johnen Str, D-52428 Julich, Germany;

    Forschungszentrum Julich, Inst Bio & Geosci, IBG Biotechnol 1, Wilhelm Johnen Str, D-52428 Julich, Germany|Rhein Westfal TH Aachen, Inst Biotechnol, Aachen, Germany;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Microalgae; Microscale; Parallelized cultivation; Scalability; Chlorella vulgaris;

    机译：微藻微尺度平行培养可扩展性小球藻;

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