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首页> 外文期刊>Bioprocess and Biosystems Engineering >Lipid accumulation and metabolic analysis based on transcriptome sequencing of filamentous oleaginous microalgae Tribonema minus at different growth phases
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Lipid accumulation and metabolic analysis based on transcriptome sequencing of filamentous oleaginous microalgae Tribonema minus at different growth phases

机译：基于转录组测序的丝状微藻Tribonema minus在不同生长阶段的脂质积累和代谢分析

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摘要

Filamentous oleaginous microalgae specie Tribonema minus is a promising feedstock for biodiesel production. However, the metabolic mechanism of lipid production in this filamentous microalgal specie remains unclear. Here, we compared the lipid accumulation of T. minus at different growth phases, and described the de novo transcriptome sequencing and assembly and identified important pathways and genes involved in TAG production. Total lipid increased by 2.5-fold and its TAG level in total lipid reached 81.1% at stationary phase. Using the genes involved in the lipid metabolism, the TAG biosynthesis pathways were generated. Moreover, results also demonstrated that, in addition to the observed overexpression of the fatty acid synthesis pathway, TAG production at stationary growth phase was bolstered by repression of the beta-oxidation pathway, up-regulation of genes that funnels acetyl-CoA to lipid biosynthesis, especially gene encoding for phospholipid:diacylglycerol acyltransferase (PDAT) which funnels DAG to TAG biosynthesis.
机译：丝状含油微藻物种Tribonema minus是一种有前途的生物柴油生产原料。然而，在这种丝状微藻物种中脂质产生的代谢机制仍不清楚。在这里，我们比较了不同生长阶段的负链球菌的脂质积累，并描述了从头转录组测序和组装，并鉴定了参与TAG产生的重要途径和基因。总脂质增加了2.5倍，在固定相中其总脂质中的TAG水平达到81.1％。使用参与脂质代谢的基因，产生TAG生物合成途径。此外，结果还表明，除了观察到的脂肪酸合成途径的过表达外，β-氧化途径的抑制，将乙酰辅酶A集中到脂质生物合成的基因的上调也促进了固定生长期的TAG产生。 ，尤其是编码磷脂：二酰基甘油酰基转移酶（PDAT）的基因，该基因使DAG进入TAG生物合成。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2017年第9期|1327-1335|共9页
  • 作者

    Wang Hui; Gao Lili; Shao Huimin; Zhou Wenjun; Liu Tianzhong;

  • 作者单位

    Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuel, Qingdao 266101, Peoples R China|Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Bioenergy Resources, Qingdao 266101, Shandong, Peoples R China;

    Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuel, Qingdao 266101, Peoples R China|Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Bioenergy Resources, Qingdao 266101, Shandong, Peoples R China;

    Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuel, Qingdao 266101, Peoples R China|Univ Chinese Acad Sci, Beijing 100049, Peoples R China;

    Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuel, Qingdao 266101, Peoples R China|Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Bioenergy Resources, Qingdao 266101, Shandong, Peoples R China;

    Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuel, Qingdao 266101, Peoples R China|Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Bioenergy Resources, Qingdao 266101, Shandong, Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Tribonema minus; Confocal image; Lipid synthesis; Transcriptome sequencing; Differential expression;

    机译：负Tribonema;共聚焦图像;脂质合成;转录组测序;差异表达;

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