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Biodiesel production potential of mixed microalgal culture grown in domestic wastewater

机译：生活污水中混合微藻培养物的生物柴油生产潜力

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摘要

In this study, a mixed microalgal culture grown in secondarily treated domestic wastewater effluent was investigated for biodiesel production using in situ transesterification method with conventional heating. The total lipid content of the mixed culture was found as 26.2 % +/- A 0.6 by weight of dry biomass, and 74 % of the lipids were contributed by total glycerides. In situ transesterification with conventional heating process under acidic conditions produced higher biodiesel yield with chloroform as the co-solvent (82.1 % +/- A 3.9) compared to hexane (55.3 % +/- A 3.9) under the same reaction conditions. The gas chromatography analysis showed that FAME composition was mainly composed of palmitic, palmitoleic, stearic, oleic, linoleic and linolenic acid methyl esters., and thus the mixed microalgal culture fed by domestic wastewaters has had comparable biodiesel conversion yields and FAME composition to mono-culture and pure cultures fed by synthetic culture media. Hence, this study showed that secondarily treated domestic wastewater could potentially be a suitable and sustainable medium for microalgae grown to be used as biodiesel feedstock.
机译：在这项研究中，使用原位酯交换法和常规加热技术，对在二次处理的生活污水中生长的混合微藻培养物进行了生物柴油生产的研究。发现混合培养物的总脂质含量为干生物质重量的26.2％+ /-0.6，并且总甘油酯贡献了74％的脂质。在相同的反应条件下，与己烷（55.3％+/- A 3.9）相比，在酸性条件下采用常规加热方法进行原位酯交换反应可产生更高的生物柴油收率，其中以氯仿作为助溶剂（82.1％+/- A 3.9）。气相色谱分析表明，FAME的组成主要由棕榈酸酯，棕榈油酸酯，硬脂酸酯，油酸，亚油酸和亚麻酸甲酯组成，因此，生活污水喂养的混合微藻培养物具有可比的生物柴油转化率和FAME组成，与单-文化和纯文化由合成培养基提供。因此，这项研究表明，二次处理的生活污水可能是适合用作微藻生长和用作生物柴油原料的可持续性培养基。

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