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Protein enrichment of brewery spent grain from Rhizopus oligosporus by solid-state fermentation

机译:固态发酵富硒根霉啤酒废谷蛋白的富集

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摘要

Brewery spent grain represents approximately 85 % of total by-products generated in a brewery. Consisting of carbohydrates, fiber, minerals and low amounts of protein, the use of brewery spent grain is limited to the feeding of ruminants; however, its potential use should be investigated. The reuse of this by-product using microorganisms by solid-state fermentation process as the case of protein enrichment by single-cell protein incorporation is an alternative to ensure sustainability and generate commercially interesting products. In this context, the aim of this study was to grow Rhizopus oligosporus in brewery spent grain under different initial moisture contents and nitrogen sources to increase the protein content of the fermented material. After 7 days of fermentation, increase of 2-4 times in the crude protein and soluble protein content was verified, respectively, compared to unfermented brewery spent grain. The kinetics of protein enrichment demonstrated the possibility of application of this technique, which can be a great alternative for use in diets for animals.
机译:啤酒厂的废谷物约占啤酒厂产生的副产品总量的85%。由碳水化合物,纤维,矿物质和少量蛋白质组成的啤酒厂废谷物的使用仅限于反刍动物的喂养;但是,应研究其潜在用途。通过固态发酵过程利用微生物再利用这种副产物,例如通过单细胞蛋白质掺入来富集蛋白质的情况,是确保可持续性和生产具有商业意义的产品的替代方法。在这种情况下,本研究的目的是在不同的初始水分含量和氮源下,在啤酒废谷物中种植低聚根霉,以增加发酵材料的蛋白质含量。发酵7天后,与未发酵的啤酒废谷物相比,粗蛋白质和可溶性蛋白质含量分别提高了2-4倍。蛋白质富集的动力学证明了应用该技术的可能性,该技术可以用作动物饮食的绝佳替代品。

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