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首页> 外文期刊>Bioprocess and Biosystems Engineering >Effect of nitrogen and/or oxygen concentration on poly (3-hydroxybutyrate) accumulation by Halomonas boliviensis
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Effect of nitrogen and/or oxygen concentration on poly (3-hydroxybutyrate) accumulation by Halomonas boliviensis

机译：氮和/或氧浓度对玻利莫氏单胞菌聚（3-羟基丁酸）积累的影响

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摘要

The behaviour of Halomonas boliviensis during growth in fed-batch culture under different kind of nutrient restrictions was examined. The metabolic switch between growth and accumulation phase is determined by the limitation in one or more essential nutrient for bacterial growth. The aim of this study was to test the effect of applying limitations of a essential nutrient, such as nitrogen, and the influence of different O-2 concentrations on poly(3-hydroxybutyrate) (PHB) production during the accumulation phase. Single limitations of nitrogen and oxygen provoke PHB accumulations of 45 and 37 % (g g(-1)), respectively, while N limitation with low O-2 supply causes the highest PHB accumulation of 73 %. The characterization of the PHB production with the strain H. boliviensis would allow a better optimization of the process and enrich the knowledge about the PHB production from strains different than Cupriavidus necator.
机译：研究了在不同营养限制下分批分批培养中玻利莫单胞菌的生长行为。生长和积累阶段之间的代谢转换取决于细菌生长的一种或多种必需营养素的限制。这项研究的目的是测试在积累阶段限制氮等基本营养素施加的影响以及不同O-2浓度对聚（3-羟基丁酸）（PHB）生产的影响。氮和氧的单一限制分别引起PHB积累45％和37％（g g（-1）），而氮限制和低O-2供给导致最高的PHB积累73％。用博氏嗜血杆菌（H. boliviensis）菌株鉴定PHB的产生将使过程更好地优化，并丰富有关来自不同于Cupravidus necator的菌株的PHB产生的知识。

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