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首页> 外文期刊>Bioprocess and Biosystems Engineering >A newly isolated and identified vitamin B-12 producing strain: Sinorhizobium meliloti 320
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A newly isolated and identified vitamin B-12 producing strain: Sinorhizobium meliloti 320

机译：新分离和鉴定出的产生维生素B-12的菌株：苜蓿中华根瘤菌320

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摘要

Vitamin B-12 (Cobalamin, VB12) has several physiological functions and is widely used in pharmaceutical and food industries. A new unicellular species was extracted from China farmland, and the strain could produce VB12 which was identified by HPLC and HPLC-MS/MS. 16S rDNA analysis reveals this strain belongs to the species Sinorhizobium meliloti and we named it S. meliloti 320. Its whole genome information indicates that this strain has a complete VB12 synthetic pathway, which paves the way for further metabolic engineering studies. The optimal carbon and nitrogen sources are sucrose and corn steep liquor (CSL) plus peptone. The optimal combination of sucrose and CSL was obtained by response surface methodology as they are the most suitable carbon and nitrogen sources, respectively. This strain could produce 140 +/- 4.2 mg L-1 vitamin B-12 after incubating for 7 days in the optimal medium.
机译：维生素B-12（Cobalamin，VB12）具有多种生理功能，被广泛用于制药和食品行业。从中国农田中提取了一种新的单细胞种，该菌株可产生VB12，通过HPLC和HPLC-MS / MS鉴定。 16S rDNA分析显示该菌株属于苜蓿中华根瘤菌，我们将其命名为S. meliloti320。其全基因组信息表明该菌株具有完整的VB12合成途径，为进一步的代谢工程研究铺平了道路。最佳的碳和氮源是蔗糖和玉米浆（CSL）加蛋白ept。通过响应面方法获得了蔗糖和CSL的最佳组合，因为它们分别是最合适的碳源和氮源。在最佳培养基中孵育7天后，该菌株可产生140 +/- 4.2 mg L-1维生素B-12。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2016年第10期|1527-1537|共11页
  • 作者

    Dong Huina; Li Sha; Fang Huan; Xia Miaomiao; Zheng Ping; Zhang Dawei; Sun Jibin;

  • 作者单位

    Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China|Chinese Acad Sci, Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China;

    Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China;

    Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China|Chinese Acad Sci, Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China;

    Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China|Chinese Acad Sci, Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China;

    Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China|Chinese Acad Sci, Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China;

    Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China|Chinese Acad Sci, Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China;

    Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China|Chinese Acad Sci, Key Lab Syst Microbial Biotechnol, Tianjin 300308, Peoples R China;

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  • 正文语种 eng
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  • 关键词

    Vitamin B-12; Sinorhizobium meliloti; Cobalamin; Whole genome sequence; Response surface methodology;

    机译：维生素B-12;苜蓿中华根瘤菌;钴胺素;全基因组序列;响应面分析法;

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