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Bioconversion of waste office paper to hydrogen using pretreated rumen fluid inoculum

机译:使用预处理的瘤胃液接种物将废办公用纸生物转化为氢气

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In this study, a microbial consortium from an acid-treated rumen fluid was used to improve the yields of H-2 production from paper residues in batch reactors. The anaerobic batch reactors, which contained paper and cellulose, were operated under three conditions: (1) 0.5 g paper/L, (2) 2 g paper/L, and (3) 4 g paper/L. Cellulase was added to promote the hydrolysis of paper to soluble sugars. The H-2 yields were 5.51, 4.65, and 3.96 mmol H-2/g COD, respectively, with substrate degradation ranging from 56 to 65.4 %. Butyric acid was the primary soluble metabolite in the three reactors, but pronounced solventogenesis was detected in the reactors incubated with increased paper concentrations (2.0 and 4.0 g/L). A substantial prevalence of Clostridium acetobutylicum (99 % similarity) was observed in the acid-treated rumen fluid, which has been recognized as an efficient H-2-producing strain in addition to ethanol and n-butanol which were also detected in the reactors.
机译:在这项研究中,使用酸处理过的瘤胃液中的微生物聚生体来提高间歇式反应器中纸渣产生H-2的产量。包含纸和纤维素的厌氧间歇反应器在以下三个条件下运行:(1)0.5 g纸/ L,(2)2 g纸/ L,和(3)4 g纸/ L。加入纤维素酶以促进纸水解成可溶性糖。 H-2的产率分别为5.51、4.65和3.96 mmol H-2 / g COD,底物降解范围为56%至65.4%。丁酸是三个反应器中的主要可溶性代谢物,但在纸浓度增加(2.0和4.0 g / L)的温育反应器中检测到明显的溶剂生成。在酸处理过的瘤胃液中观察到了丙酮丁酸梭菌的大量流行(99%相似性),除了在反应器中也检测到的乙醇和正丁醇外,该瘤胃液还被认为是产生H-2的有效菌株。

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