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首页> 外文期刊>Bioprocess and Biosystems Engineering >Enhanced production of 2,3-butanediol by a genetically engineered Bacillus sp. BRC1 using a hydrolysate of empty palm fruit bunches
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Enhanced production of 2,3-butanediol by a genetically engineered Bacillus sp. BRC1 using a hydrolysate of empty palm fruit bunches

机译：基因工程芽孢杆菌属物种提高了2,3-丁二醇的生产。 BRC1使用空棕榈果束的水解产物

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摘要

A Bacillus species that produces 2,3-butanediol (2,3-BD), termed BRC1, was newly isolated, and a 2,3-BD dehydrogenase (Bdh) from this species was identified and characterized at the molecular and biochemical level. Sequence analysis revealed that Bdh is homologous to D-2,3-BD dehydrogenases. An analysis of the enzymatic properties of Bdh overexpressed in Escherichia coli confirmed the molecular results, showing preferred activity toward D-2,3-BD. Optimum pH, temperature, and kinetics determined for reductive and oxidative reactions support the preferential production of 2,3-BD during cell growth. Overexpression of bdh under the control of a xylose-inducible promoter resulted in increased enzyme activity and enhanced 2,3-BD production in Bacillus sp. BRC1. Additionally, a hydrolysate of cellulosic material, (empty palm fruit bunches), was successfully used for the enhanced production of 2,3-BD in the recombinant Bacillus strain.
机译：新分离出了生产2,3-丁二醇（2,3-BD）的芽孢杆菌物种，称为BRC1，并从该物种中鉴定了2,3-BD脱氢酶（Bdh）并在分子和生化水平上进行了表征。序列分析表明，Bdh与D-2，3-BD脱氢酶同源。对在大肠杆菌中过表达的Bdh的酶学性质的分析证实了分子结果，显示了对D-2,3-BD的优选活性。为还原和氧化反应确定的最佳pH，温度和动力学支持在细胞生长期间优先产生2,3-BD。在木糖诱导型启动子的控制下，bdh的过度表达导致芽孢杆菌中酶活性增加和2,3-BD产量增加。 BRC1。此外，纤维素材料（空棕榈果串）的水解产物已成功用于增强重组芽孢杆菌菌株中2,3-BD的生产。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2015年第2期|299-305|共7页
  • 作者

    In Yeong Kang; Jang Min Park; Won-Kyung Hong; Yun Seok Kim; You Ree Jung; Seung-Bum Kim; Sun-Yeon Heo; Sung-Mok Lee; Ji Young Kang; Baek-Rock Oh; Dae-Hyuk Kim; Jeong-Woo Seo; Chul Ho Kim;

  • 作者单位

    Biorefinery Research Center, Jeonbuk Branch Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup, Jeonbuk 580-185, South Korea,Department of Bioactive Materials, College of Natural Sciences, Chonbuk National University, Jeonju, Chonbuk 561-756, South Korea;

    Biorefinery Research Center, Jeonbuk Branch Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup, Jeonbuk 580-185, South Korea;

    Biorefinery Research Center, Jeonbuk Branch Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup, Jeonbuk 580-185, South Korea;

    Biorefinery Research Center, Jeonbuk Branch Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup, Jeonbuk 580-185, South Korea;

    Biorefinery Research Center, Jeonbuk Branch Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup, Jeonbuk 580-185, South Korea;

    Biorefinery Research Center, Jeonbuk Branch Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup, Jeonbuk 580-185, South Korea;

    Biorefinery Research Center, Jeonbuk Branch Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup, Jeonbuk 580-185, South Korea;

    Biorefinery Research Center, Jeonbuk Branch Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup, Jeonbuk 580-185, South Korea;

    Biorefinery Research Center, Jeonbuk Branch Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup, Jeonbuk 580-185, South Korea;

    Biorefinery Research Center, Jeonbuk Branch Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup, Jeonbuk 580-185, South Korea;

    Department of Bioactive Materials, College of Natural Sciences, Chonbuk National University, Jeonju, Chonbuk 561-756, South Korea;

    Biorefinery Research Center, Jeonbuk Branch Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup, Jeonbuk 580-185, South Korea;

    Biorefinery Research Center, Jeonbuk Branch Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup, Jeonbuk 580-185, South Korea;

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  • 关键词

    Bacillus sp.; 2,3-butanediol; 2,3-butanediol dehydrogenase; Empty palm fruit bunches;

    机译：芽孢杆菌2,3-丁二醇;2,3-丁二醇脱氢酶;空棕榈果串;

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