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首页> 外文期刊>Bioprocess and Biosystems Engineering >Optimization of the integrated citric acid-methane fermentation process by air stripping and glucoamylase addition
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Optimization of the integrated citric acid-methane fermentation process by air stripping and glucoamylase addition

机译:通过空气汽提和添加葡糖淀粉酶优化柠檬酸-甲烷综合发酵工艺

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摘要

To solve the problem of extraction wastewater in citric acid industry, an integrated citric acid-methane fermentation process was proposed. In the integrated process, extraction wastewater was treated by mesophilic anaerobic digestion and then reused to make mash for the next batch of citric acid fermentation. In this study, an Aspergillus niger mutant strain exhibiting resistance to high metal ions concentration was used to eliminate the inhibition of 200 mg/L Na~+ and 300 mg/L K~+ in anaerobic digestion effluent (ADE) and citric acid production increased by 25.0 %. Air stripping was used to remove ammonium, alkalinity, and part of metal ions in ADE before making mash. In consequence, citric acid production was significantly improved but still lower by 6.1 % than the control. Results indicated that metal ions in ADE synergistically inhibited the activity of glucoamylase, thus reducing citric acid production. When 130 U/g glucoamylase was added before fermentation, citric acid production was 141.5 g/L, which was even higher than the control (140.4 g/L). This process could completely eliminate extraction wastewater discharge and reduce water resource consumption.
机译:为了解决柠檬酸工业中提取废水的问题,提出了柠檬酸-甲烷综合发酵工艺。在集成过程中,提取废水经过中温厌氧消化处理,然后再用于制取浆,用于下一批柠檬酸发酵。在这项研究中,对高金属离子浓度具有抗性的黑曲霉突变菌株用于消除200 mg / L Na〜+和300 mg / LK〜+对厌氧消化液(ADE)的抑制作用,柠檬酸产量增加25.0%。制浆之前,使用空气汽提除去ADE中的铵,碱度和部分金属离子。结果,柠檬酸的产量显着提高,但仍比对照低6.1%。结果表明,ADE中的金属离子协同抑制了葡糖淀粉酶的活性,从而降低了柠檬酸的产生。发酵前添加130 U / g葡糖淀粉酶时,柠檬酸产量为141.5 g / L,甚至高于对照(140.4 g / L)。该过程可以完全消除提取废水的排放,减少水资源消耗。

著录项

  • 来源
    《Bioprocess and Biosystems Engineering》 |2015年第3期|411-420|共10页
  • 作者单位

    The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China;

    The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China;

    The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China;

    The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China;

    The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China;

    The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China;

    The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China;

    The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Citric acid; Anaerobic digestion; Air stripping; Metal ions; Glucoamylase;

    机译:柠檬酸;厌氧消化;空气汽提;金属离子;葡糖淀粉酶;

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