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首页> 外文期刊>Bioprocess and Biosystems Engineering >Utilization of orange peel, a food industrial waste, in the production of exo-polygalacturonase by pellet forming Aspergillus sojae
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Utilization of orange peel, a food industrial waste, in the production of exo-polygalacturonase by pellet forming Aspergillus sojae

机译：食品工业废料橘皮在颗粒状大豆曲霉生产外切多聚半乳糖醛酸酶中的利用

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摘要

The production of exo-polygalacturonase (exo-PG) from orange peel (OP), a food industrial waste, using Aspergillus sojae was studied in submerged culture. A simple, low-cost, industrially significant medium formulation, composed of only OP and (NH_4)_2SO_4 (AS) was developed. At an inoculum size of 2.8 × 10~3 spores/mL, growth was in the form of pellets, which provided better mixing of the culture broth and higher exo-PG activity. These pellets were successfully used as an inoculum for bioreactors and 173.0 U/mL exo-PG was produced. Fed-batch cultivation further enhanced the exo-PG activity to 244.0 U/mL in 127.5 h. The final morphology in the form of pellets is significant to industrial fermentation easing the subsequent downstream processing. Furthermore, the low pH trend obtained during this fermentation serves an advantage to fungal fermentations prone to contamination problems. As a result, an economical exo-PG production process was defined utilizing a food industrial byproduct and producing high amount of enzyme.
机译：在深水培养中研究了使用大豆酱曲霉从食品工业废料橙皮（OP）中生产外聚半乳糖醛酸酶（exo-PG）的过程。开发了一种仅由OP和（NH_4）_2SO_4（AS）组成的简单，低成本，工业上重要的培养基配方。接种量为2.8×10〜3孢子/ mL时，以小球形式生长，这提供了更好的培养液混合和更高的exo-PG活性。这些沉淀物已成功用作生物反应器的接种物，并生产了173.0 U / mL exo-PG。分批补料培养在127.5小时内将exo-PG活性进一步提高至244.0 U / mL。颗粒形式的最终形态对工业发酵很重要，从而简化了后续的下游加工过程。此外，在该发酵过程中获得的低pH趋势对于易于产生污染问题的真菌发酵具有优势。结果，定义了一种经济的exo-PG生产方法，其利用食品工业副产物并产生大量的酶。

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