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首页> 外文期刊>Bioprocess and Biosystems Engineering >Bioreduction of U(Ⅵ) in groundwater under anoxic conditions from a decommissioned in situ leaching uranium mine
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Bioreduction of U(Ⅵ) in groundwater under anoxic conditions from a decommissioned in situ leaching uranium mine

机译：退役原位浸出铀矿在缺氧条件下对地下水中U（Ⅵ）的生物还原

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摘要

To determine whether the U(Ⅵ) in ground-water under anoxic conditions at a decommissioned in situ leaching (ISL) uranium mine could be bioreduced, groundwater samples containing suspended sediments were taken from the mine, experimental setup was fabricated, and the jar containing the groundwater in the setup was amended with ethanol and incubated under anoxic conditions. The variations of pH, chemical oxygen demand, nitrate, sulfate, U(Ⅵ), and dissolved oxygen (DO) concentrations were monitored during the incubation. U(Ⅵ) concentration dropped to 0.043 mg/L when the stimulated microorganisms were active, and it then increased to 0.835 mg/L within 10 days after the metabolism of the stimulated microorganisms was inhibited. The DO variation was observed in the amended jar during the incubation, and the metabolism of the stimulated microorganisms was found to affect the DO concentration. Firmicutes were found to be dominant in the sediments in the amended jar through the 16S rRNA pyrosequencing. The results indicate that it is possible to bioreduce U(Ⅵ) in the ground-water under anoxic conditions at the decommissioned ISL uranium mine by adding carbon source into it without removing the oxygen from it.
机译：为了确定是否可以对退役的原位浸出（ISL）铀矿在缺氧条件下的地下水中的U（Ⅵ）进行生物还原，从该矿中采集了包含悬浮沉积物的地下水样品，制作了实验装置，并盛装了装有该罐的罐子装置中的地下水用乙醇修正，并在缺氧条件下孵育。在孵育过程中监测pH，化学需氧量，硝酸盐，硫酸盐，U（Ⅵ）和溶解氧（DO）浓度的变化。当被刺激的微生物处于活跃状态时，U（Ⅵ）的浓度降至0.043 mg / L，在被刺激的微生物的代谢被抑制后的10天内，U（Ⅵ）的浓度升高至0.835 mg / L。在温育期间，在修改过的广口瓶中观察到DO的变化，并且发现受刺激微生物的代谢会影响DO的浓度。通过16S rRNA焦磷酸测序，发现在修改过的广口瓶中的沉积物中占主导地位的是硬菌。结果表明，在退役的ISL铀矿中，在缺氧条件下，可以通过向其中添加碳源而不去除氧气来生物还原地下水中的U（Ⅵ）。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2015年第4期|661-669|共9页
  • 作者

    De-xin Ding; Shi-mi Li; Nan Hu; Fei Xu; Guang-yue Li; Yong-dong Wang;

  • 作者单位

    Key Discipline Laboratory for National Defense for Biotechnology in Uranium Mining and Hydrometallurgy, University of South China, 28 West Changsheng Road, Hengyang 421001, Hunan, People's Republic of China;

    Key Discipline Laboratory for National Defense for Biotechnology in Uranium Mining and Hydrometallurgy, University of South China, 28 West Changsheng Road, Hengyang 421001, Hunan, People's Republic of China;

    Key Discipline Laboratory for National Defense for Biotechnology in Uranium Mining and Hydrometallurgy, University of South China, 28 West Changsheng Road, Hengyang 421001, Hunan, People's Republic of China;

    Key Discipline Laboratory for National Defense for Biotechnology in Uranium Mining and Hydrometallurgy, University of South China, 28 West Changsheng Road, Hengyang 421001, Hunan, People's Republic of China;

    Key Discipline Laboratory for National Defense for Biotechnology in Uranium Mining and Hydrometallurgy, University of South China, 28 West Changsheng Road, Hengyang 421001, Hunan, People's Republic of China;

    Key Discipline Laboratory for National Defense for Biotechnology in Uranium Mining and Hydrometallurgy, University of South China, 28 West Changsheng Road, Hengyang 421001, Hunan, People's Republic of China;

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  • 正文语种 eng
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  • 关键词

    In situ leaching; Groundwater; Bioreduction; U(Ⅵ); Anaerobic zone;

    机译：原位浸出;地下水;生物还原U（Ⅵ）;厌氧区;

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