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首页> 外文期刊>Bioprocess and Biosystems Engineering >Purification and characterization of cyclodextrin β-glucanotransferase from novel alkalophilic bacilli
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Purification and characterization of cyclodextrin β-glucanotransferase from novel alkalophilic bacilli

机译：新型嗜碱杆菌的环糊精β-葡糖基转移酶的纯化与鉴定

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摘要

The discovery of novel bacterial cyclodextrin glucanotransferase (CGTase) enzyme could provide advantages in terms of its production and relative activity. In this study, eight bacterial strains isolated from soils of a biodiversity-rich vegetation in Egypt based on their hydrolyzing activity of starch, were screened for CGTase activity, where the most active strain was identified as Bacillus lehensis. Optimization process revealed that the using of rice starch (25 %) and a mixture of peptone/yeast extract (1 %) at pH 10.5 and 37 ℃ for 24 h improved the bacterial growth and enzyme activity. The bacterial CGTase was successively purified by acetone precipitation, gel filtration chromatography in a Sephadex G-100 column and ion exchange chromatography in a DEAE-cellulose column. The specific activity of the CGTase was increased approximately 274-fold, from 0.21 U/mg protein in crude broth to 57.7 U/mg protein after applying the DEAE-cellulose column chromatography. SDS-PAGE showed that the purified CGTase was homogeneous with a molecular weight of 74.1 kDa. Characterization of the enzyme exhibited optimum pH and temperature of 7 and 60 ℃, respectively. CGTase relative activity was strongly inhibited by Mg~(2+), Zn~(2+), Al~(3+) and K~+, while it was slightly enhanced by 5 and 9 % with Cu~(2+) and Fe~(2+) metal ions, respectively.
机译：新型细菌环糊精葡聚糖转移酶（CGTase）酶的发现可以提供其生产和相对活性方面的优势。在这项研究中，根据淀粉的水解活性，从埃及生物多样性丰富的植被的土壤中分离出的八种细菌菌株进行了CGTase活性筛选，其中最活跃的菌株被鉴定为Lehensis。优化过程表明，使用大米淀粉（25％）和蛋白one /酵母提取物的混合物（1％）在pH 10.5和37℃下混合24h可以改善细菌的生长和酶活性。依次通过丙酮沉淀，Sephadex G-100柱中的凝胶过滤色谱法和DEAE-纤维素柱中的离子交换色谱法依次纯化细菌CGTase。在应用DEAE纤维素柱色谱法后，CGTase的比活性从粗肉汤中的0.21 U / mg蛋白增加到约57.7 U / mg蛋白，增加了约274倍。 SDS-PAGE显示纯化的CGTase是均质的，分子量为74.1kDa。酶的最佳pH值分别为7和60℃。 CGTase相对活性受到Mg〜（2 +），Zn〜（2 +），Al〜（3+）和K〜+的强烈抑制，而Cu〜（2+）和K〜+分别略微提高了5％和9％。 Fe〜（2+）金属离子。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2015年第4期|767-776|共10页
  • 作者

    Ashraf F. Elbaz; Ahmed Sobhi; Ahmed ElMekawy;

  • 作者单位

    Genetic Engineering and Biotechnology Research Institute, University of Sadat City (USC), Sadat City, Egypt,Chemical and Biomedical Engineering Department, Ohio State University (OSU), Columbus, OH, USA;

    Genetic Engineering and Biotechnology Research Institute, University of Sadat City (USC), Sadat City, Egypt;

    Genetic Engineering and Biotechnology Research Institute, University of Sadat City (USC), Sadat City, Egypt;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Bacillus; Cyclodextrin P-glucanotransferase; Purification; Starch;

    机译：芽孢杆菌环糊精P-葡聚糖转移酶;纯化;淀粉;

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