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Toxic effect of environmentally relevant concentration of silver nanoparticles on environmentally beneficial bacterium Pseudomonas putida

机译:环境相关浓度的银纳米颗粒对环境有益细菌恶臭假单胞菌的毒性作用

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摘要

Silver nanoparticles (Ag NPs) are being increasingly used in many consumer products owing to their excellent antimicrobial properties. The continuous use of Ag NPs in consumer products will lead to environmental release. The present study evaluated the toxic effects and the possible underlying mechanism of Ag NPs on Pseudomonas putida. Ag NP exposure inhibited growth of the cells. Increased lipid peroxidation occurred coincident with suppression of the antioxidant defense system. Ag NP exposure caused reactive oxygen species (ROS) production, glutathione depletion and inactivation of the antioxidant enzyme superoxide dismutase, catalase and glutathione reductase. The addition of superoxide dismutase or pretreatment of P. putida with N-acetyl cysteine that quenches ROS reduced toxicity of the NPs.
机译:银纳米颗粒(Ag NPs)由于其优异的抗菌性能而越来越多地用于许多消费产品中。 Ag NP在消费产品中的持续使用将导致环境释放。本研究评估了银纳米颗粒对恶臭假单胞菌的毒性作用及其可能的潜在机制。 Ag NP暴露抑制了细胞的生长。脂质过氧化增加与抗氧化防御系统的抑制同时发生。 Ag NP暴露引起活性氧(ROS)的产生,谷胱甘肽耗竭以及抗氧化酶超氧化物歧化酶,过氧化氢酶和谷胱甘肽还原酶的失活。添加超氧化物歧化酶或用N-乙酰半胱氨酸淬灭ROS预处理恶臭假单胞菌可降低NP的毒性。

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