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首页> 外文期刊>Bioprocess and Biosystems Engineering >Streptomyces sp. LK3 mediated synthesis of silver nanoparticles and its biomedical application
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Streptomyces sp. LK3 mediated synthesis of silver nanoparticles and its biomedical application

机译：链霉菌LK3介导的纳米银的合成及其生物医学应用

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摘要

In the present study, the marine actinobacteria mediated biosynthesis of silver nanoparticles (AgNps) was achieved using Streptomyces sp LK3. The synthesized AgNps showed the characteristic absorption spectra in UV-vis at 420 nm, which confirmed the presence of nanoparticles. XRD analysis showed intense peaks at 29 values of 27.51°, 31.87°, 45.57°, 56.56°, 66.26°, and 75.25° corresponding to (210), (113), (124), (240), (226), and (300) Bragg's reflection based on the fcc structure of AgNps. The FTIR spectra exhibited prominent peaks at 3,417 cm~(-1) (OH stretching due to alcoholic group) and 1,578 cm~(-1) (C=C ring stretching). TEM micrograph showed that the synthesized AgNps were spherical in shape with an average size of 5 nm. Surface morphology and topographical structure of the synthesized AgNps were dignified by AFM. The synthesized AgNps showed significant acaricidal activity against Rhipicephalus microplus and Haemaphysalis bispinosa with LC_(50) values of 16.10 and 16.45 mg/L, respectively. Our results clearly indicate that AgNps could provide a safer alternative to conventional acaricidal agents in the form of a topical antiparasitic formulation. The present study aimed to develop a novel, cost-effective, eco-friendly actinobacteria mediated synthesis of AgNps and its antiparasitic activity.
机译：在本研究中，海洋链霉菌介导的银纳米颗粒（AgNps）的生物合成是使用链霉菌sp LK3实现的。合成的AgNps在420 nm的紫外可见光谱中表现出特征吸收光谱，这证实了纳米颗粒的存在。 XRD分析显示强烈峰出现在27.51°，31.87°，45.57°，56.56°，66.26°和75.25°的29个值处，分别对应于（210），（113），（124），（240），（226）和（300）布拉格基于AgNps的fcc结构的反射。 FTIR光谱在3,417 cm〜（-1）（由于醇基团引起的OH拉伸）和1,578cm〜（-1）（C = C环拉伸）处表现出突出的峰。 TEM显微照片显示，合成的AgNps为球形，平均大小为5nm。用原子力显微镜（AFM）对合成的AgNps的表面形态和形貌结构进行了鉴定。合成的AgNps表现出对微小头R和双头血吸虫的显着杀螨活性，LC_（50）值分别为16.10和16.45 mg / L。我们的结果清楚地表明，AgNps可以局部抗寄生虫制剂的形式提供比常规杀螨剂更安全的替代品。本研究旨在开发一种新型的，具有成本效益的，环保的放线菌介导的AgNps合成及其抗寄生虫活性。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2014年第2期|261-267|共7页
  • 作者

    L. Karthik; Gaurav Kumar; A. Vishnu Kirthi; A. A. Rahuman; K. V. Bhaskara Rao;

  • 作者单位

    Environmental Biotechnology Division, School of Bio Sciences and Technology, VIT University, Vellore 632 014,Tamil Nadu, India;

    Environmental Biotechnology Division, School of Bio Sciences and Technology, VIT University, Vellore 632 014,Tamil Nadu, India;

    Unit of Nanotechnology and Bioactive Natural Products, Post Graduate and Research Department of Zoology, C. Abdul Hakeem College, Melvisharam, Vellore 632 509, Tamil Nadu, India;

    Unit of Nanotechnology and Bioactive Natural Products, Post Graduate and Research Department of Zoology, C. Abdul Hakeem College, Melvisharam, Vellore 632 509, Tamil Nadu, India;

    Environmental Biotechnology Division, School of Bio Sciences and Technology, VIT University, Vellore 632 014,Tamil Nadu, India;

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  • 正文语种 eng
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  • 关键词

    AFM; Silver nanoparticles; Streptomyces sp LK3; TEM; XRD;

    机译：原子力显微镜银纳米颗粒;链霉菌sp LK3;TEM;X射线衍射;

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