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首页> 外文期刊>Bioprocess and Biosystems Engineering >Enzymatic liquefaction and saccharification of pretreated corn stover at high-solids concentrations in a horizontal rotating bioreactor
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Enzymatic liquefaction and saccharification of pretreated corn stover at high-solids concentrations in a horizontal rotating bioreactor

机译：卧式旋转生物反应器中高固体浓度预处理玉米秸秆的酶促液化和糖化作用

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摘要

A self-designed horizontal rotating bioreactor (HRR) was applied for enzymatic hydrolysis of pretreated corn stover to improve the process economics of ethanol production. The mixing principle was based on gravity and free fall employed with tank-rotating. The liquefaction performances using the HRR and the vertical stirred-tank reactor (VSTR) with a helical impeller were compared and analyzed by measuring rheological properties of the slurry. During the enzymatic hydrolysis, viscosity decreased dramatically in the initial phase for both bioreactors and more pronouncedly for the HRR. Rheological parameters fitted to the power law showed that shear thinning properties of the slurry weakened during the reaction. The glucose concentration was used to define the efficiency of the saccharification reaction. The HRR also proved to be more efficient for glucose release with both the constant and fed-batch substrate addition modes. Liquefaction and saccharification at 25 % w/w dry matter (DM) and enzyme loading of 7 FPU/g DM resulted in the optimal glucose concentration of 86 g/kg. Results revealed a decrease in cellulose conversion at increasing initial DM, which was slighter in the HRR compared with that in the VSTR.
机译：将自行设计的卧式旋转生物反应器（HRR）用于酶解预处理的玉米秸秆，以提高乙醇生产的工艺经济性。混合原理基于重力和罐旋转时的自由落体。通过测量浆液的流变特性，比较和分析了使用HRR和带有螺旋叶轮的立式搅拌釜反应器（VSTR）的液化性能。在酶促水解过程中，两种生物反应器的初始粘度均显着下降，而HRR则更为明显。符合幂律的流变参数表明，反应过程中浆料的剪切稀化性能减弱。葡萄糖浓度用于定义糖化反应的效率。在恒定和补料分批添加底物的模式下，HRR还被证明对释放葡萄糖更有效。在25％w / w干物质（DM）下的液化和糖化作用以及7 FPU / g DM的酶负载量导致最佳葡萄糖浓度为86 g / kg。结果显示，在初始DM升高时，纤维素转化率降低，与VSTR相比，HRR的降低幅度较小。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2014年第2期|173-181|共9页
  • 作者

    Jian Du; Fazhan Zhang; Yuanyuan Li; Hongman Zhang; Jingrui Liang; Hongbo Zheng; He Huang;

  • 作者单位

    College of Life Science and Pharmacy, Nanjing University of Technology, Nanjing 210009, Jiangsu, China;

    College of Science, Nanjing University of Technology,Nanjing 210009, Jiangsu, China;

    College of Science, Nanjing University of Technology,Nanjing 210009, Jiangsu, China;

    College of Science, Nanjing University of Technology,Nanjing 210009, Jiangsu, China;

    College of Science, Nanjing University of Technology,Nanjing 210009, Jiangsu, China;

    College of Science, Nanjing University of Technology,Nanjing 210009, Jiangsu, China;

    College of Life Science and Pharmacy, Nanjing University of Technology, Nanjing 210009, Jiangsu, China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Corn stover; Bioreactor; Enzymatic hydrolysis; Rheology;

    机译：玉米秸秆;生物反应器;酶促水解;流变学;

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