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首页> 外文期刊>Bioprocess and Biosystems Engineering >Production and optimization of poly-γ-glutamic acid by Bacillus subtilis BL53 isolated from the Amazonian environment
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Production and optimization of poly-γ-glutamic acid by Bacillus subtilis BL53 isolated from the Amazonian environment

机译：从亚马逊环境分离的枯草芽孢杆菌BL53生产和优化聚-γ-谷氨酸

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The aims of this research were to screen and characterize a new microbial source of y-PGA, to optimize aspects of culture conditions and medium composition using central composite design and response surface methodologies. The influence of bioreactor stirring rates on the production of γ-PGA was also investigated and the oxygen volumetric mass transfer coefficients (k_La) were established. The most productive strain was identified by 16S rDNA analysis as Bacillus subtilis, and its γ-PGA production in rotatory shaker was threefold increased under optimized conditions (37 ℃, pH 6.9, and 1.22 mM Zn~(2+)), compared to conventional medium. In bioreactor, the γ-PGA production was further increased, reaching 17 g 1~(-1), 70 ％ higher than shaker cultures. γ-PGA production showed high dependency on oxygen transfer. At K_La of 210 h~(-1) the cultivation time could be reduced to 48 h, about 50 ％ of the time required for operations at K_La 55 h(-1).
机译：这项研究的目的是筛选和表征新型的y-PGA微生物来源，使用集中复合设计和响应面方法优化培养条件和培养基成分。还研究了生物反应器搅拌速率对γ-PGA产生的影响，并建立了氧气体积传质系数（k_La）。通过16S rDNA分析鉴定出最高产菌株为枯草芽孢杆菌，在最佳条件（37℃，pH 6.9和1.22 mM Zn〜（2+））下，旋转摇床中γ-PGA的产量增加了三倍。介质。在生物反应器中，γ-PGA的产量进一步增加，达到17 g 1〜（-1），比摇床培养物高70％。 γ-PGA的生产显示出高度依赖于氧气的转移。在210 h〜（-1）的K_La下，培养时间可以减少到48 h，大约是在55 h（-1）K_La下操作所需时间的50％。

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