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首页> 外文期刊>Bioprocess and Biosystems Engineering >Mixed carbon source control strategy for enhancing alginate lyase production by marine Vibrio sp. QY102
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Mixed carbon source control strategy for enhancing alginate lyase production by marine Vibrio sp. QY102

机译：混合碳源控制策略可增强海洋弧菌产生藻酸盐裂解酶的能力。 QY102

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摘要

Medium and culture conditions for alginate lyase production by marine Vibrio sp. QY102 were first optimized using statistical methods including Plackett-Burman design and central composite design. Then, fermentation in 5-L bioreactor showed that alginate acted as easily used carbohydrate for Vibrio sp. QY102, while starch extended its growth phase and stabilized pH variations. Thus, a novel strategy using mixed carbon sources was proposed that starch supported growth while enzyme synthesis was induced by pulse feedings of solid alginate. The optimized process followed that Vibrio sp. QY102 grew on starch until the end of the logarithmic growth phase, and then solid alginate was added as 1 g/L every 3 h. Meanwhile, initial pH 5.0 and natural pH during fermentation was favorable for alginate lyase production. After optimization, the highest alginate lyase production reached 52.8 U/mL, which was 329 ％ higher than the control. Finally, fermentation scale-up was performed in 30-L bioreactor and the maximum alginate lyase production was obtained as 46.8 U/mL.
机译：海洋弧菌生产海藻酸裂解酶的培养基和培养条件。首先使用统计方法（包括Plackett-Burman设计和中央复合设计）对QY102进行优化。然后，在5-L生物反应器中发酵表明藻酸盐可作为Vibrio sp的易用碳水化合物。 QY102，而淀粉延长其生长期并稳定pH值变化。因此，提出了使用混合碳源的新策略，即淀粉支持生长，而通过固体藻酸盐的脉冲进料诱导酶合成。经过优化的过程是Vibrio sp。 QY102在淀粉上生长直至对数生长期结束，然后每3小时以1 g / L的速度添加固体藻酸盐。同时，发酵过程中的初始pH 5.0和自然pH有利于藻酸盐裂解酶的产生。经过优化，藻酸盐裂解酶的最高产量达到52.8 U / mL，比对照高329％。最后，在30-L生物反应器中进行发酵放大，获得的最大藻酸盐裂解酶产量为46.8 U / mL。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2014年第3期|575-584|共10页
  • 作者

    Jiushun Zhou; Menghao Cai; Tao Jiang; Weiqiang Zhou; Wei Shen; Xiangshan Zhou; Yuanxing Zhang;

  • 作者单位

    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China;

    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China;

    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China;

    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China;

    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China;

    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China;

    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Alginate induction; Alginate lyase; Vibrio sp. QY102; Medium optimization; Mixed carbon source;

    机译：海藻酸钠诱导;海藻酸裂解酶;弧菌QY102;中等优化;混合碳源;

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