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首页> 外文期刊>Bioprocess and Biosystems Engineering >Cancerous cell targeting and destruction using pH stabilized amperometric bioconjugated gold nanoparticles from marine macroalgae, Padina gymnospora
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Cancerous cell targeting and destruction using pH stabilized amperometric bioconjugated gold nanoparticles from marine macroalgae, Padina gymnospora

机译:使用来自海洋大型藻类(Padina Gymnospora)的pH稳定的安培生物共轭金纳米颗粒对癌细胞进行靶向和破坏

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摘要

The main aim of this study was, using bio-mechanistic approach, to synthesize and characterize amperometric stable gold nanoparticles (AuNPs) under different pH conditions using UV Spec, dynamic light scattering and TEM with energy dispersive X-ray analysis. The biomolecules involved in conjugation and reduction were further characterized by Fourier transform infrared analysis. The pH stabilized nanoparticles were studied to determine the functional and molecular mechanism of cell death on liver cancer (HepG2) cell line and gastric cancer (YCC3) cell line. The zeta potential and TEM imaging demonstrated that AuNPs were spherical in nature and can pass through the cellular membrane because of their intrinsic properties of AuNPs to bind to carbon-bonded sulfhydryl (-C-SH or R-SH) group and, therefore, could interact with intracellular components of the cell which was confirmed through phase contrast microscopy. Altered molecular mechanism and cellular effects in different cancer cell suggest a potential for in vivo applications of gold nanomaterials.
机译:这项研究的主要目的是使用生物力学方法,使用UV光谱,动态光散射和TEM和能量色散X射线分析法,在不同pH条件下合成和表征安培稳定的金纳米颗粒(AuNPs)。通过傅立叶变换红外分析进一步表征了参与缀合和还原的生物分子。研究了pH稳定的纳米颗粒,以确定细胞死亡对肝癌(HepG2)和胃癌(YCC3)细胞系的功能和分子机制。 Zeta电位和TEM成像表明,AuNPs呈球形,可通过细胞膜,因为AuNPs具有与碳键巯基(-C-SH或R-SH)结合的固有特性,因此可以通过相差显微镜确认与细胞的细胞内成分发生相互作用。在不同癌细胞中改变的分子机制和细胞效应提示了金纳米材料在体内应用的潜力。

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