...
首页> 外文期刊>Bioprocess and Biosystems Engineering >Screening and characterization of Sphingomonas sp. mutant for welan gum biosynthesis at an elevated temperature
【24h】

Screening and characterization of Sphingomonas sp. mutant for welan gum biosynthesis at an elevated temperature

机译:鞘氨醇单胞菌的筛选和鉴定。高温下Welan胶生物合成的突变体

获取原文
获取原文并翻译 | 示例
           

摘要

The optimal temperature for the microbial polysaccharide fermentation is no higher than 30 ℃, which is economically undesirable due to additional cooling cost. To solve this problem in the case of welan gum production, we obtained the high-temperature-tolerant-producing strain, Sphingomonas sp. HT-1 by atmospheric and room-temperature plasma-induced mutation. Using HT-1, we obtained a concentration and 1 % aqueous viscosity of 26.8 ± 0.34 g/L and 3.50 ± 0.05 Pa s at a comparatively higher optimal temperature (37 ℃). HT-1 was further characterized to understand the mechanism by which these properties are improved. Results indicated that high yield could be attributed to the following: (1) enhanced intra-cellular synthesis, demonstrated by an increase in the activities of key enzymes, and (2) accelerated cross-membrane substrate uptake and product secretion, indicated by improved membrane fluidity and permeability. Temperature tolerance could be attributed to the overex-pression of the investigated heat shock proteins and oxi-dative stress proteins.
机译:微生物多糖发酵的最佳温度不高于30℃,由于额外的冷却成本,在经济上是不希望的。为了在生产Welan口香糖的情况下解决该问题,我们获得了产生高温的菌株Sphingomonas sp。 HT-1受大气和室温等离子体诱导的突变。使用HT-1,我们在相对较高的最佳温度(37℃)下获得了26.8±0.34 g / L和3.50±0.05 Pa s的浓度和1%的水粘度。 HT-1被进一步表征以了解改善这些特性的机理。结果表明,高产量可能归因于以下原因:(1)增强的细胞内合成,表现为关键酶活性的增加;(2)加速的跨膜底物吸收和产物分泌,表现为膜的改善流动性和渗透性。温度耐受性可以归因于所研究的热激蛋白和氧化应激蛋白的过表达。

著录项

  • 来源
    《Bioprocess and Biosystems Engineering》 |2014年第9期|1849-1858|共10页
  • 作者单位

    State Key Laboratory of Materials-Oriented Chemical Engineering, College of Food Science and Light Industry, Nanjing University of Technology, No. 30 South Puzhu Road, Pukou District, Nanjing 211816, China;

    State Key Laboratory of Materials-Oriented Chemical Engineering, College of Food Science and Light Industry, Nanjing University of Technology, No. 30 South Puzhu Road, Pukou District, Nanjing 211816, China;

    State Key Laboratory of Materials-Oriented Chemical Engineering, College of Food Science and Light Industry, Nanjing University of Technology, No. 30 South Puzhu Road, Pukou District, Nanjing 211816, China;

    State Key Laboratory of Materials-Oriented Chemical Engineering, College of Food Science and Light Industry, Nanjing University of Technology, No. 30 South Puzhu Road, Pukou District, Nanjing 211816, China;

    State Key Laboratory of Materials-Oriented Chemical Engineering, College of Food Science and Light Industry, Nanjing University of Technology, No. 30 South Puzhu Road, Pukou District, Nanjing 211816, China;

    State Key Laboratory of Materials-Oriented Chemical Engineering, College of Food Science and Light Industry, Nanjing University of Technology, No. 30 South Puzhu Road, Pukou District, Nanjing 211816, China;

    Hebei Xinhe Biochemical Corporation Limited, No. 10 Xinlong Street, Chengxi Industrial Zone, Xinhe County 055650, Hebei, China;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Atmospheric pressure glow discharge; Welan gum; Biosynthesis; Enzyme activity; Membrane fluidity;

    机译:大气压辉光放电;威兰胶;生物合成;酶活性;膜流动性;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号