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l-Lactic acid production benefits from reduction of environmental osmotic stress through neutralizing agent combination

机译：通过中和剂的组合减少环境渗透胁迫可生产l-乳酸

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摘要

This paper hinged on the combination effect of two different neutralizing agents Ca(OH)_2 and NH_4OH on the production of l-lactic acid by Lactobacillus paracasei. Present study quantitatively indicated that environmental osmotic pressure (844-1,772 mOsm/kg) exerted minor influence on l-lactic acid production, but a critical level fell on approximately 3,000 mOsm/kg which restricted l-lactic acid production significantly. Once osmotic pressure exceeded 3,600 mOsm/kg, l-lactic acid production ran aground. A new and efficient neutralizing agent-adding strategy was established in this study to procure 2.21-fold enhancement (5.94 g/l/h) relative to previous productivity of l-lactic acid with NH_4OH as neutralizing agent via batch cultivation. It was, therefore, speculated that inhibition effect in the late phase of the fermentation might be in large part attributed to the dramatic increase of environmental osmotic stress, other than cumulative effect of lactate concentration itself.
机译：本文探讨了两种不同的中和剂Ca（OH）_2和NH_4OH对副干酪乳杆菌生产L-乳酸的联合作用。目前的研究定量地表明，环境渗透压（844-1,772 mOsm / kg）对l-乳酸的产生影响较小，但临界水平下降到大约3,000 mOsm / kg，这严重限制了l-乳酸的产生。一旦渗透压超过3,600 mOsm / kg，就产生了l-乳酸。在这项研究中，建立了一种新的有效的中和剂添加策略，通过分批培养，相对于以前的以NH_4OH作为中和剂的l-乳酸生产率，可提高2.21倍（5.94 g / l / h）。因此，据推测，发酵后期的抑制作用可能很大程度上归因于环境渗透压的急剧增加，而不是乳酸浓度本身的累积作用。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2014年第9期|1917-1923|共7页
  • 作者

    Xiwei Tian; Yonghong Wang; Ju Chu; Yingping Zhuang; Siliang Zhang;

  • 作者单位

    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, P.O. Box 329, Shanghai 200237, People's Republic of China;

    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, P.O. Box 329, Shanghai 200237, People's Republic of China;

    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, P.O. Box 329, Shanghai 200237, People's Republic of China;

    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, P.O. Box 329, Shanghai 200237, People's Republic of China;

    State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, P.O. Box 329, Shanghai 200237, People's Republic of China;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    Lactobacillus paracasei; l-Lactic acid production; Neutralizing agent; Osmotic pressure;

    机译：副干酪乳杆菌;l-乳酸生产;中和剂;渗透压;

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