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Recovery of electric energy from formate by using a recombinant strain of Escherichia coli

机译:使用重组大肠杆菌从甲酸盐中回收电能

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摘要

Recombinant Escherichia coli cells were applied for the recovery of electric energy from formate. Initially, the fdh gene, which encodes formate dehydroge-nase (FDH) of Mycobacterium vaccae, was introduced into E. coli cells to allow efficient degradation of formate. The constructed microbial fuel cell (MFC) with E. coli BW25113 cells carrying fdh gene showed appreciable generation of current density in the presence of formate as a substrate. Current density and polarization curves revealed that the performance of MFC under examined conditions was limited by the electron transfer from bulk liquid to the electrode surface; accordingly, agitation resulted in an increase in the current density and achieved a coulombic efficiency of 21.7 % on the basis of formate consumed. Thus, gene recombination enables E. coli cells to utilize formate as a fuel for MFC.
机译:重组大肠杆菌细胞用于从甲酸盐中回收电能。最初,将编码牛痘分枝杆菌的甲酸脱氢酶(FDH)的fdh基因引入大肠杆菌细胞,以实现甲酸的有效降解。带有fdh基因的大肠杆菌BW25113细胞构建的微生物燃料电池(MFC)在甲酸作为底物的情况下显示出可观的电流密度产生。电流密度和极化曲线表明,在所考察的条件下,MFC的性能受到电子从本体液体向电极表面转移的限制。因此,搅拌导致电流密度增加,并且基于所消耗的甲酸盐,库仑效率达到了21.7%。因此,基因重组使大肠杆菌细胞能够利用甲酸作为MFC的燃料。

著录项

  • 来源
    《Bioprocess and Biosystems Engineering》 |2014年第10期|2005-2008|共4页
  • 作者单位

    Division of Chemical Engineering, Department of Materials Engineering Science, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka,Osaka 560-8531, Japan;

    Division of Chemical Engineering, Department of Materials Engineering Science, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka,Osaka 560-8531, Japan;

    Division of Chemical Engineering, Department of Materials Engineering Science, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka,Osaka 560-8531, Japan;

    Division of Chemical Engineering, Department of Materials Engineering Science, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka,Osaka 560-8531, Japan;

    Division of Chemical Engineering, Department of Materials Engineering Science, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka,Osaka 560-8531, Japan;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Microbial fuel cell; Escherichia coli; Formate; Formate dehydrogenase;

    机译:微生物燃料电池;大肠杆菌;格式;甲酰胺脱氢酶;

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