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首页> 外文期刊>Bioprocess and Biosystems Engineering >In vivo and in vitro decolorization of synthetic dyes by laccase from solid state fermentation with Trametes sp. SYBC-L4
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In vivo and in vitro decolorization of synthetic dyes by laccase from solid state fermentation with Trametes sp. SYBC-L4

机译：用Trametes sp。固态发酵的漆酶对合成染料进行体内和体外脱色。 SYBC-L4

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摘要

Synthetic decolorization of dyes through solid cassava residue substrate fermentation with Trametes sp. SYBC-L4 via in vivo and in vitro processes was investigated in this study. Effects of pH and mediator (1-hydroxybenzotriazole, HBT) concentration on dyes decolorization were evaluated. In vitro, decolorization ratios of dyes differed considerably in pH and increased with the increasing of HBT concentration. Crude laccase (50 U/L) derived from Trametes sp. SYBC-L4 decolorized 67.91 ± 1.25 % Congo red (100 mg/L), 94.58 ± 1.05 % aniline blue (100 mg/L) and 99.02 ± 0.54 % indigo carmine (100 mg/L) with 2.5 mM HBT at pH 4.5 in 36 h of incubation. In vivo, decolorization ratios of dyes were not enhanced by usage of the mediator. After 10 days of fermentation, decolorization ratio of Congo red (1,000 mg/kg), aniline blue (1,000 mg/kg) and indigo carmine (1,000 mg/kg) was 57.82 ± 0.84, 92.53 ± 1.12 and 97.26 ± 1.92 % without the usage of mediator at pH 4.5, respectively. Moreover, there was no obvious difference between the in vivo decolorization of aniline blue and indigo carmine in the pH range of 3.0-9.0. Results showed that Trametes sp. SYBC-L4 had great potential to be used for dyes decolorization via in vivo and in vitro processes. Moreover, in terms of pH range and mediator, in vivo decolorization with Trametes sp. SYBC-L4 was more advantageous since laccase mediator was needless and the applicable range of pH was broader.
机译：通过Trametes sp。通过固体木薯残渣底物发酵对染料进行合成脱色。 SYBC-L4通过体内和体外的过程进行了研究。评估了pH和介质（1-羟基苯并三唑，HBT）浓度对染料脱色的影响。在体外，染料的脱色率在pH值上相差很大，并且随着HBT浓度的增加而增加。来自Trametes sp。的粗漆酶（50 U / L）。 SYBC-L4的脱色为67.91±1.25％的刚果红（100 mg / L），94.58±1.05％的苯胺蓝（100 mg / L）和99.02±0.54％的靛蓝胭脂红（100 mg / L）和2.5 mM HBT（pH 4.5）孵育36小时。在体内，通过使用介体不能提高染料的脱色率。发酵10天后，刚果红（1,000 mg / kg），苯胺蓝（1,000 mg / kg）和靛蓝胭脂红（1,000 mg / kg）的脱色率分别为57.82±0.84、92.53±1.12和97.26±1.92％。分别在pH 4.5下使用介体。此外，在3.0-9.0的pH范围内，苯胺蓝和靛蓝胭脂红的体内脱色之间没有明显差异。结果表明，Trametes sp。 SYBC-L4具有很大的潜力，可用于通过体内和体外过程使染料脱色。此外，就pH范围和介体而言，Trametes sp。的体内脱色。 SYBC-L4更有利，因为漆酶介体是不必要的，pH的适用范围更广。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2014年第12期|2597-2605|共9页
  • 作者

    Hui-Xing Li; Rui-Jing Zhang; Lei Tang; Jian-Hua Zhang; Zhong-Gui Mao;

  • 作者单位

    The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, People's Republic of China,School of Biological and Chemical Engineering, Nanyang Institute of Technology, Nanyang 473004, People's Republic of China;

    The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, People's Republic of China;

    The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, People's Republic of China;

    The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, People's Republic of China;

    The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, People's Republic of China;

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  • 正文语种 eng
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  • 关键词

    Laccase; Dyes decolorization; In vitro; In vivo; Mediator;

    机译：漆酶;染料脱色;体外;体内;调解员;

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