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首页> 外文期刊>Bioprocess and Biosystems Engineering >Kinetics of sucrose conversion to fructo-oligosaccharides using enzyme (invertase) under free condition
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Kinetics of sucrose conversion to fructo-oligosaccharides using enzyme (invertase) under free condition

机译：在自由条件下使用酶（转化酶）将蔗糖转化为低聚果糖的动力学

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The study reports the synthesis of fructo-oli-gosaccharide (FOS) from sucrose using invertase derived from Saccharomyces cerevisiae. The reaction was conducted in a batch mode under free enzyme condition. Fructo-oligosaccharide formation was detected at a high sucrose concentration of over 200 g/L. The investigation was extended to study the effect of different parameters such as initial sucrose concentration (ISC), pH, and enzyme concentration. A maximum FOS yield of 10 % (dry basis) was observed using 525 g/L of ISC, with 6 U/ mL of the enzyme, and pH 5.5 at 40 ℃. 1-Kestose was the major product of among different forms of FOS. The FOS yield increased with an increase in sucrose concentration up to 525 g/L, beyond which it started to decrease. However, the maximum FOS yield was not affected by the increasing concentration of the enzyme beyond a certain level (2 U/mL). Furthermore, the activity of enzyme slightly increased with an increase in the pH up to 6, and thereafter it declined. Addition of glucose decreased the FOS yield because of enzyme inhibition. A five-step, ten-parameter model was developed, for which the simulation was performed in COPASI. The results predicted by the model were consistent with the experimental data.
机译：该研究报道了使用源自酿酒酵母的转化酶从蔗糖合成果糖寡糖（FOS）。反应在游离​​酶条件下以间歇方式进行。在超过200 g / L的高蔗糖浓度下检测到低聚果糖。这项研究扩展到研究不同参数的影响，例如初始蔗糖浓度（ISC），pH和酶浓度。使用525 g / L的ISC，6 U / mL的酶和40℃的pH 5.5观察到最大FOS产率为10％（干基）。 1-Koseose是不同形式的FOS中的主要产品。 FOS产量随蔗糖浓度增加至525 g / L而增加，然后开始下降。但是，最大FOS产量不受酶浓度增加超过一定水平（2 U / mL）的影响。此外，随着pH的增加，酶的活性略有增加，直至pH升高至6，此后下降。由于酶抑制，添加葡萄糖降低了FOS的产量。开发了一个五步，十参数的模型，为此在COPASI中进行了仿真。该模型预测的结果与实验数据一致。

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