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首页> 外文期刊>Bioprocess and Biosystems Engineering >Bioethanol production from brown seaweed, Undaria pinnatifida,using NaCl acclimated yeast
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Bioethanol production from brown seaweed, Undaria pinnatifida,using NaCl acclimated yeast

机译：使用NaCl驯化酵母从褐海藻Undaria pinnatifida生产生物乙醇

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Strain improvement of Pichia angophorae KCTC 17574 was successfully carried out for bioethanol fermentation of seaweed slurry with high salt concentration. P. angophorae KCTC 17574 was cultured under increasing salinity from five practical salinity unit (psu, %o) to as high as 100 psu for 723 h. The seaweed, Undaria pinnatifida (sea mustard, Miyuk), was fermented to produce bioethanol using high-salt acclimated yeast. The pretreatment of U. pinnatifida was optimized using thermal acid hydrolysis to obtain a high monosaccharide yield. Optimal pretreatment conditions of 75 mM H_2SO_4 and 13 % (w/v) slurry at 121 ℃ for 60 min were determined using response surface methodology. A maximum monosaccharide content of 28.65 g/L and the viscosity of 33.19 cP were obtained. The yeasts cultured under various salinity concentrations were collected and inoculated to the pretreated seaweed slurry after the neutralization using 5 N NaOH. The pretreated slurry was fermented with the inoculation of 0.1 g dcw/L of P. angophorae KCTC 17574 strain obtained at 90 psu. The maximum ethanol concentration of 9.42 g/L with 27 % yield of theoretical case of ethanol production from total carbohydrate of U. pinnatifida was obtained.
机译：成功地进行了毕赤酵母KCTC 17574的菌株改良，以用于高盐浓度海藻浆液的生物乙醇发酵。 P. angophorae KCTC 17574在盐度从五个实用盐度单位（psu，％o）升高至最高100 psu的条件下培养723小时。使用高盐适应性酵母发酵海藻Undaria pinnatifida（海芥，Miyuk），以生产生物乙醇。 pinnatifida的预处理使用热酸水解进行了优化，以获得高单糖收率。采用响应面法确定了75 mM H_2SO_4和13％（w / v）浆料在121℃下60 min的最佳预处理条件。获得的最大单糖含量为28.65 g / L，粘度为33.19 cP。收集在不同盐度浓度下培养的酵母，并在用5 N NaOH中和后接种到预处理的海藻浆中。接种0.1 g dcw / L的P. angophorae KCTC 17574菌株，发酵得到的浆液在90 psu接种。获得最大乙醇浓度为9.42g / L，理论上从pinnatifida的总碳水化合物生产乙醇的情况下产率为27％。

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