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首页> 外文期刊>Bioprocess and Biosystems Engineering >Modification of response behavior of zinc sensing HydHG two-component system using a self-activation loop and genomic integration
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Modification of response behavior of zinc sensing HydHG two-component system using a self-activation loop and genomic integration

机译：使用自激活环和基因组整合修改锌感测HydHG两组分系统的响应行为

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摘要

Characterizing the dynamics of HydHG-a two-component transcriptional regulatory network for exogenous zinc in E. coli-is essential in understanding the biology of these regulatory and signaling pathways. Here, we used a synthetic biology strategy to modify the dynamic characteristics of the HydHG network in two ways. First, a self-activation loop for HydHG network was created under the control of zraP promoter, after which the threshold Zn~(2+) concentration for the self-activated HydHG network significantly decreased from 200 to 10 μM. Second, the self-activation loop was integrated into the E. coli genome allowing the threshold Zn~(2+) concentration to be elevated to 500 μM. As the threshold Zn~(2+) concentration could be modified in both directions, the introduction of a self-activation loop and the entire genomic integration strategy may prove useful for the creation of a two-component bacterial biosensor with varying sensitivities.
机译：表征HydHG（大肠杆菌中外源锌的两组分转录调控网络）的动力学特性对于理解这些调控和信号传导途径的生物学至关重要。在这里，我们使用合成生物学策略以两种方式修改HydHG网络的动态特性。首先，在zraP启动子的控制下建立了HydHG网络的自激活环，此后，自激活HydHG网络的阈值Zn〜（2+）浓度从200μM显着降低至10μM。其次，将自激活环整合到大肠杆菌基因组中，使Zn〜（2+）阈值浓度提高到500μM。由于可以在两个方向上修改阈值Zn〜（2+）浓度，因此引入自激活环和整个基因组整合策略可能对创建具有不同灵敏度的双组分细菌生物传感器很有用。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2013年第9期|1185-1190|共6页
  • 作者

    Van Dung Pham; Sambandam Ravikumar; Seung Hwan Lee; Soon Ho Hong; Ik-Keun Yoo;

  • 作者单位

    School of Chemical Engineering and Bioengineering,University of Ulsan, 93 Daehakro, Nam-gu, Ulsan 680-749,Republic of Korea;

    School of Chemical Engineering and Bioengineering,University of Ulsan, 93 Daehakro, Nam-gu, Ulsan 680-749,Republic of Korea;

    Chemical Biotechnology Research Center,Korea Research Institute of Chemical Technology,Yuseong-gu, Daejeon 305-600, Republic of Korea;

    School of Chemical Engineering and Bioengineering,University of Ulsan, 93 Daehakro, Nam-gu, Ulsan 680-749,Republic of Korea;

    School of Chemical Engineering and Bioengineering,University of Ulsan, 93 Daehakro, Nam-gu, Ulsan 680-749,Republic of Korea;

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  • 正文语种 eng
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  • 关键词

    Escherichia coli; HydHG two-component system; zraP promoter; Zinc; Self-activation loop;

    机译：大肠杆菌;HydHG两组分系统;zraP启动子;锌;自激活循环;

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