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首页> 外文期刊>Bioprocess and Biosystems Engineering >Correlation between dielectric property by dielectrophoretic levitation and growth activity of cells exposed to electric field
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Correlation between dielectric property by dielectrophoretic levitation and growth activity of cells exposed to electric field

机译：介电悬浮的介电性能与暴露于电场的细胞生长活性之间的相关性

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摘要

The purpose of this study is to develop a system analyzing cell activity by the dielectrophoresis method. Our previous studies revealed a correlation between the growth activity and dielectric property (Re[K(ω)]) of mouse hybridoma 3-2H3 cells using dielectrophoretic levitation. Furthermore, it was clarified that the differentiation activity of many stem cells could be evaluated by the Re[K(ω)] without differentiation induction. In this paper, 3-2H3 cells exposed to an alternating current (AC) electric field or a direct current (DC) electric field were cultivated, and the influence of damage by the electric field on the growth activity of the cells was examined. To evaluate the activity of the cells by measuring the Rc[K(ω)], the correlation between the growth activity and the Re[K(ω)] of the cells exposed to the electric field was examined. The relations between the cell viability, growth activity, and Re[K(ω)] in the cells exposed to the AC electric field were obtained. The growth activity of the cells exposed to the AC electric field could be evaluated by the Re[K(ω)]. Furthermore, it was found that the adverse effects of the electric field on the cell viability and the growth activity were smaller in the AC electric field than the DC electric field.
机译：这项研究的目的是开发一种通过介电电泳法分析细胞活性的系统。我们以前的研究表明，使用介电泳悬浮法，小鼠杂交瘤3-2H3细胞的生长活性与介电特性（Re [K（ω）]）之间存在相关性。此外，阐明了可以通过Re [K（ω）]评估许多干细胞的分化活性而无需分化诱导。在本文中，培养了暴露于交流（AC）电场或直流（DC）电场的3-2H3细胞，并研究了电场损伤对细胞生长活性的影响。为了通过测量Rc [K（ω）]来评估细胞的活性，检查了生长活性和暴露于电场的细胞的Re [K（ω）]之间的相关性。获得了暴露于交流电场的细胞中细胞活力，生长活性和Re [K（ω）]之间的关系。可以通过Re [K（ω）]评估暴露于交流电场的细胞的生长活性。此外，发现电场对细胞活力和生长活性的不利影响在交流电场中比在直流电场中小。

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