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首页> 外文期刊>Bioprocess and Biosystems Engineering >Approaching chlorpyrifos bioelimination at bench scale bioreactor
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Approaching chlorpyrifos bioelimination at bench scale bioreactor

机译：在台式生物反应器中接近毒死rif生物消除

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Chlorpyrifos (CP) is one of the most commonly applied insecticides for control of pests and insects. The inappropriate use of this kind of chemicals has caused heavy contamination of many terrestrial and aquatic ecosystems thus representing a great environmental and health risk. The main purpose of this work is to investigate novel microbial agents (Pseudomonas stutzeri and the previously obtained consortium LB2) with the ability to degrade CP from polluted effluents. This goal was achieved by operating at different lab scales (flask and bioreactor) and operation modes (batch and fed-batch). Very low degradation and biomass levels were detected in cultures performed with the consortium LB2. In contrast, near complete CP degradation was reached by P. stutzeri at the optimal conditions in less than 1 month, showing a depletion rate of 0.054 h~(-1). The scale-up at bench scale stirred tank bioreactor allowed improving the specific degradation rate in ten folds and total CP degradation was obtained after 2 days. Moreover, biomass and biodegradation profiles were modelled to reach a better characterization of the bioremediation process.
机译：毒死rif（CP）是控制害虫和昆虫的最常用杀虫剂之一。此类化学药品的不当使用已导致许多陆地和水生生态系统受到严重污染，从而带来巨大的环境和健康风险。这项工作的主要目的是研究能够降解受污染废水中CP的新型微生物制剂（施氏假单胞菌和先前获得的财团LB2）。通过在不同的实验室规模（烧瓶和生物反应器）和操作模式（分批和补料分批）下运行来实现此目标。在用财团LB2进行的培养中检测到非常低的降解和生物量水平。相反，在最佳条件下，斯图氏假丝酵母在不到1个月的时间内达到了接近完全的CP降解，显示出0.054 h〜（-1）的消耗速率。在台式规模的搅拌槽生物反应器上进行放大可将比降解速率提高十倍，并在2天后获得总CP降解。此外，对生物质和生物降解特性进行了建模，以更好地表征生物修复过程。

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