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首页> 外文期刊>Bioprocess and Biosystems Engineering >Identification and characterization of Klebsiella pneumoniae aldehyde dehydrogenases increasing production of 3-hydroxypropionic acid from glycerol
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Identification and characterization of Klebsiella pneumoniae aldehyde dehydrogenases increasing production of 3-hydroxypropionic acid from glycerol

机译：肺炎克雷伯氏菌醛脱氢酶的鉴定和表征，可提高甘油中3-羟基丙酸的产量

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摘要

Klebsiella pneumoniae produces 3-hydroxypropionic acid (3-HP) from glycerol with oxidation of 3-hydroxypropionaldehyde (3-HPA) to 3-HP in a reaction catalyzed by aldehyde dehydrogenase (ALDH). In the present study, two putative ALDHs of K. pneumoniae, Ynel and YdcW were identified and characterized. Recombinant Ynel was specifically active on 3-HPA and preferred NAD~+ as a cofactor, whereas YdcW exhibited broad substrate specificity and preferred NADP~+ as a cofactor. Overexpression of ALDHs in the glycerol oxi-dative pathway-deficient mutant K. pneumoniae AK resulted in a significant increase in 3-HP production upon shake-flask culture. The final titers of 3-HP were 2.4 and 1.8 g L~(-1) by recombinants overexpressing Ynel and YdcW, respectively. Deletion of the ALDH gene from K. pneumoniae did not affect the extent of 3-HP synthesis, implying non-specific activity of ALDHs on 3-HPA. The ALDHs might play major roles in detoxifying the aldehyde generated in glycerol metabolism.
机译：在乙醛脱氢酶（ALDH）催化的反应中，肺炎克雷伯菌由甘油产生3-羟基丙酸（3-HP），并将3-羟基丙醛（3-HPA）氧化为3-HP。在本研究中，鉴定和表征了两个推定的肺炎克雷伯菌ALDH，Ynel和YdcW。重组Ynel对3-HPA具有特异性活性，优选作为辅助因子的NAD- +，而YdcW表现出广泛的底物特异性，优选作为辅助因子的NADP- +。甘油氧化还原途径缺陷型肺炎克雷伯氏菌AK中ALDHs的过度表达导致摇瓶培养中3-HP产量的显着增加。通过过量表达Ynel和YdcW的重组体，3-HP的最终效价分别为2.4和1.8 g L〜（-1）。从肺炎克雷伯菌中删除ALDH基因不影响3-HP合成的程度，这暗示ALDH对3-HPA的非特异性活性。 ALDHs在解毒甘油代谢中产生的醛方面可能起主要作用。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2013年第9期|1319-1326|共8页
  • 作者

    Lian Hua Luo; Jeong-Woo Seo; Sun-Yeon Heo; Baek-Rock Oh; Dae-Hyuk Kim; Chul Ho Kim;

  • 作者单位

    Applied Microbiology Research Center, Bio-Materials Research Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup-si 580-185, South Korea,Institute for Molecular Biology and Genetics, Research Center of Bioactive Materials, Chonbuk National University,Jeonju-si 561-756, South Korea;

    Applied Microbiology Research Center, Bio-Materials Research Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup-si 580-185, South Korea;

    Applied Microbiology Research Center, Bio-Materials Research Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup-si 580-185, South Korea;

    Applied Microbiology Research Center, Bio-Materials Research Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup-si 580-185, South Korea;

    Institute for Molecular Biology and Genetics, Research Center of Bioactive Materials, Chonbuk National University,Jeonju-si 561-756, South Korea;

    Applied Microbiology Research Center, Bio-Materials Research Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup-si 580-185, South Korea;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    Glycerol; 3-Hydroxypropionic acid; Klebsiella pneumoniae; Aldehyde dehydrogenase;

    机译：甘油;3-羟基丙酸;肺炎克雷伯菌;醛脱氢酶;

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