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首页> 外文期刊>Bioprocess and Biosystems Engineering >Conversion of carbon dioxide to oxaloacetate using integrated carbonic anhydrase and phosphoenolpyruvate carboxylase
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Conversion of carbon dioxide to oxaloacetate using integrated carbonic anhydrase and phosphoenolpyruvate carboxylase

机译：使用整合的碳酸酐酶和磷酸烯醇丙酮酸羧化酶将二氧化碳转化为草酰乙酸

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摘要

The development and implementation of strategies for CO_2 mitigation are necessary to counteract the greenhouse gas effect of carbon dioxide emissions. To demonstrate the possibility of simultaneously capturing CO_2 and utilizing four-carbon compounds, an integrated system using CA and PEPCase was developed, which mimics an in vivo carbon dioxide concentration mechanism. We first cloned the PEPCase 1 gene of the marine diatom Phaeodactylum tricornutum and produced a recombinant PtPEPCase 1. The affinity column purified PtPEPCase 1 exhibited specific enzymatic activity (5.89 U/mg). When the simultaneous and coordinated reactions of CA from Dunaliella sp. and the PtPEPCase 1 occurred, more OAA was produced than when only PEPCase was present. Therefore, this integrated CA-PEPCase system can be used not only to capture CO_2 but also for a new technology to produce value-added four-carbon platform chemicals.
机译：制定和实施减少CO_2的策略对于抵消二氧化碳排放对温室气体的影响是必要的。为了证明同时捕获CO_2和利用四碳化合物的可能性，开发了使用CA和PEPCase的集成系统，该系统模仿了体内二氧化碳的浓缩机制。我们首先克隆了海生硅藻三角藻（Phaeodactylum tricornutum）的PEPCase 1基因，并产生了重组PtPEPCase1。亲和柱纯化的PtPEPCase 1表现出特定的酶活性（5.89 U / mg）。当杜氏藻的CA同时发生并协调反应时。并且发生PtPEPCase 1时，比仅存在PEPCase时产生更多的OAA。因此，这种集成的CA-PEPCase系统不仅可以用于捕获CO_2，还可以用于生产增值四碳平台化学品的新技术。

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