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Improvement of hydrocarbon recovery by spouting solvent into culture of Botryococcus braunii

机译：通过喷射溶剂培养布鲁氏葡萄球菌提高烃采收率

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摘要

Botryococcus braunii, a green microalga, is known to produce plentiful liquid hydrocarbons as promising biodiesel resources. However, the hydrocarbon extraction methods that have so far achieved have several problems such as low efficiency and high cost. In our study, a solvent-spouted extraction process integrated with photo-bioculture was designed for simultaneous realization of hydrocarbon extraction and cell culture in two phases. The n-octane was selected as the best solvent among several solvents because its biocompatibility was highest for B. braunii. As a result, high level of biomass and hydrocarbon, 4.17 and 893.79 mg/L, respectively, was attained at 100 mL/min of solvent recycling rate through three times of processes for 66 days. Moreover, formation of cell clump was suppressed in solvent extraction, cells were regenerated after it, and thus cell viability was maintained even after repeated cycles of it. Finally, this solvent-spouted culture process required the smaller cost due to reuse of the less solvent and regenerated cells, compared with the other conventional methods. Accordingly, this technique would be applicable to exploit the continuous extraction of hydrocarbon from the algal biomass, especially for application on a large scale.
机译：众所周知，布氏葡萄球菌是一种绿色的微藻，可产生大量液态烃，是有前途的生物柴油资源。然而，迄今为止已经实现的烃提取方法具有诸如效率低和成本高的几个问题。在我们的研究中，设计了一种与光生物培养法相结合的溶剂喷射萃取工艺，可同时实现烃萃取和细胞培养两个阶段。选择正辛烷作为几种溶剂中最好的溶剂，因为它对布鲁氏双歧杆菌的生物相容性最高。结果，在三天的过程中，以100mL / min的溶剂回收速率，经过66天的三遍处理，分别获得了4.17和893.79 mg / L的高水平生物质和碳氢化合物。此外，在溶剂萃取中抑制了细胞团的形成，其后细胞得以再生，因此即使在重复其循环之后也保持了细胞活力。最后，与其他常规方法相比，由于溶剂较少的再生细胞的重复使用，这种溶剂喷射培养方法所需的成本更低。因此，该技术将适用于从藻类生物质中连续提取烃，特别是大规模应用。

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