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首页> 外文期刊>Bioprocess and Biosystems Engineering >Kinetic models of ribonucleic acid fermentation and continuous culture by Candida tropicalis no.121
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Kinetic models of ribonucleic acid fermentation and continuous culture by Candida tropicalis no.121

机译：121号热带假丝酵母核糖核酸发酵和连续培养的动力学模型

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摘要

During ribonucleic acid fermentation, the fermentative processes were researched at pH controlled at 4.0 and under natural conditions. Unstructured models in a 50-L airlift fermentor were established for batch RNA production at pH 4.0 using the Verhulst equation for microbial growth, the Luedeking-Piret equation for product formation and a Luedeking-Piret-like equation for substrate uptake. Parameters of the kinetic models were determined using origin 7.5. Based on the models estimated above, another batch fermentation experiment was conducted in a 300-L airlift fermentor, which demonstrated that the models could be useful for RNA production on an industrial scale. Additionally, continuous fermentation based on kinetic models was proposed to make full use of substrates and reduce the cost of waste water treatment. As a result, although the DCW and RNA concentration were 11.5 and 1.68 g L~', which were lower than that of batch fermentation, the sugar utilization increased by 14.3%, while the waste water decreased by more than 90%.
机译：在核糖核酸发酵过程中，研究了在pH值控制在4.0和自然条件下的发酵过程。使用Verhulst方程用于微生物生长，Luedeking-Piret方程用于产物形成，Luedeking-Piret方程用于底物吸收，在50 L气举发酵罐中建立非结构化模型，以在pH 4.0下批量生产RNA。使用原点7.5确定动力学模型的参数。基于上述模型，在300升气举发酵罐中进行了另一批发酵实验，证明该模型可用于工业规模的RNA生产。另外，提出了基于动力学模型的连续发酵以充分利用底物并降低废水处理成本。结果，尽管DCW和RNA浓度分别为11.5和1.68g L-，比分批发酵的要低，但是糖的利用率增加了14.3％，而废水减少了90％以上。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2012年第3期|p.415-422|共8页
  • 作者

    Bingbing Li; Xiaochun Chen; Huajing Ren; Lei Li; Jian Xiong; Jianxin Bai; Yong Chen; Jinglan Wu; Hanjie Ying;

  • 作者单位

    State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, People's Republic of China;

    State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, People's Republic of China;

    State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, People's Republic of China;

    State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, People's Republic of China;

    State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, People's Republic of China;

    State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, People's Republic of China;

    State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, People's Republic of China;

    State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, People's Republic of China;

    State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, People's Republic of China;

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  • 正文语种 eng
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  • 关键词

    candida tropicalis no.121; continuous fermentation; kinetic model; ribonucleic acid;

    机译：热带假丝酵母121号;连续发酵动力学模型核糖核酸;

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