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首页> 外文期刊>Bioprocess and Biosystems Engineering >Use of sugarcane molasses 'B' as an alternative for ethanol production with wild-type yeast Saccharomyces cerevisiae ITV-01 at high sugar concentrations
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Use of sugarcane molasses 'B' as an alternative for ethanol production with wild-type yeast Saccharomyces cerevisiae ITV-01 at high sugar concentrations

机译：在高糖浓度下使用甘蔗糖蜜“ B”替代野生型酿酒酵母ITV-01生产乙醇

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摘要

Molasses "B" is a rich co-product of the sugarcane process. It is obtained from the second step of crystallization and is richer in fermentable sugars (50-65%) than the final molasses, with a lower non-sugar solid content (18-33%); this co-product also contains good vitamin and mineral levels. The use of molasses "B" for ethanol production could be a good option for the sugarcane industry when cane sugar prices diminish in the market. In a complex medium like molasses, osmotoler-ance is a desirable characteristic for ethanol producing strains. The aim of this work was to evaluate the use of molasses "B" for ethanol production using Saccharomyces cerevisiae ITV-01 (a wild-type yeast isolated from sugarcane molasses) using different initial sugar concentrations (70-291 g L~(-1)), two inoculum sizes and the addition of nutrients such as yeast extract, urea, and ammonium sulphate to the culture medium. The results obtained showed that the strain was able to grow at 291 g L~(-1) total sugars in molasses "B" medium; the addition of nutrients to the culture medium did not produce a statistically significant difference. This yeast exhibits high osmotolerance in this medium, producing high ethanol yields (0.41 g g~(-1)). The best conditions for ethanol production were 220 g L~(-1) initial total sugars in molasses "B" medium, pH 5.5, using an inoculum size of 6 x 10~6 cell mL~(-1); ethanol production was 85 g L~(-1), productivity 3.8 gL~(-1)h~(-1) with 90% preserved cell viability.
机译：糖蜜“ B”是甘蔗工艺的丰富副产品。它是从第二步结晶获得的，比最终的糖蜜富含可发酵的糖（50-65％），非糖固体含量较低（18-33％）；该副产品还含有良好的维生素和矿物质。当甘蔗糖市场价格下降时，将糖蜜“ B”用于乙醇生产可能是甘蔗行业的一个不错的选择。在像糖蜜这样的复杂培养基中，渗透压是产乙醇菌株的理想特性。这项工作的目的是使用不同的初始糖浓度（70-291 g L〜（-1），评估糖蜜“ B”在酿酒酵母ITV-01（一种从甘蔗糖蜜中分离的野生型酵母）的乙醇生产中的应用。 ）），两种接种物的大小以及向培养基中添加营养物质（例如酵母提取物，尿素和硫酸铵）的能力。结果表明，该菌株能够在糖蜜“ B”培养基中以291 g L〜（-1）的总糖量生长。向培养基中添加营养素没有产生统计学上的显着差异。该酵母在这种培养基中表现出高渗透压耐受性，产生高乙醇产量（0.41 g g（-1））。乙醇生产的最佳条件是220 g L〜（-1）糖蜜“ B”培养基（pH 5.5）中的初始总糖，接种量为6 x 10〜6细胞mL〜（-1）。乙醇产量为85 g L〜（-1），产量为3.8 gL〜（-1）h〜（-1），细胞活力得以保留90％。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2012年第4期|p.605-614|共10页
  • 作者

    C. L. Fernandez-Lopez; B. Torrestiana-Sanchez; M. A. Salgado-Cervantes; P. G. Mendoza Garcia; M. G. Aguilar-Uscanga;

  • 作者单位

    Instituto Tecnologico de Veracruz, UNIDA (Unidad de Investigacion y Desarrollo en Alimentos), Bio-Engineering Laboratory, Av. Miguel A. de Quevedo 2779, Col. Formando Hogar, CP 91860 Veracruz, Ver., Mexico;

    Instituto Tecnologico de Veracruz, UNIDA (Unidad de Investigacion y Desarrollo en Alimentos), Bio-Engineering Laboratory, Av. Miguel A. de Quevedo 2779, Col. Formando Hogar, CP 91860 Veracruz, Ver., Mexico;

    Instituto Tecnologico de Veracruz, UNIDA (Unidad de Investigacion y Desarrollo en Alimentos), Bio-Engineering Laboratory, Av. Miguel A. de Quevedo 2779, Col. Formando Hogar, CP 91860 Veracruz, Ver., Mexico;

    Instituto Tecnologico de Veracruz, UNIDA (Unidad de Investigacion y Desarrollo en Alimentos), Bio-Engineering Laboratory, Av. Miguel A. de Quevedo 2779, Col. Formando Hogar, CP 91860 Veracruz, Ver., Mexico;

    Instituto Tecnologico de Veracruz, UNIDA (Unidad de Investigacion y Desarrollo en Alimentos), Bio-Engineering Laboratory, Av. Miguel A. de Quevedo 2779, Col. Formando Hogar, CP 91860 Veracruz, Ver., Mexico;

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  • 正文语种 eng
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  • 关键词

    ethanol; molasses "B"; osmotolerance inoculum size;

    机译：乙醇;糖蜜“ B”;耐渗透菌大小;

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