...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Bioprocess and Biosystems Engineering >Effects of carbon sources and feeding strategies on heparosan production by Escherichia coli K5
【24h】

Effects of carbon sources and feeding strategies on heparosan production by Escherichia coli K5

机译：碳源和饲养策略对大肠杆菌K5产生肝素的影响

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

This work aimed to develop an optimal carbon source feeding strategy to achieve maximal production of heparosan as a precursor of bioengineered heparin by Escherichia coli K5. Glycerol gave higher heparosan titer and productivity compared to glucose. The maximum heparosan production (187 mg/L) and heparosan productivity (5.19 mg/L/h) in glycerol-defined medium were 26.4% higher than the heparosan production (148 mg/L) and heparosan productivity (4.11 mg/L/h) in glucose-defined medium. DO-stat feeding approach as compared to pH-stat feeding, exponential feeding, exponential combined with pH-stat feeding, and constant rate feeding gave the highest heparosan titer at 8.63 g/L, which was nine times that of batch culture. The obtained optimal glycerol feeding strategy may be useful for the scaling-up of microbial heparosan production.
机译：这项工作旨在开发一种最佳的碳源补给策略，以实现大肠杆菌K5最大限度地生产作为生物工程肝素前体的肝素。与葡萄糖相比，甘油的肝素滴度和生产率更高。在甘油定义的培养基中，最大肝素产量（187 mg / L）和肝素生产率（5.19 mg / L / h）比肝素产量（148 mg / L）和肝素生产率（4.11 mg / L / h）高26.4％ ）在葡萄糖定义的培养基中。与pH恒定进料，指数添加，指数结合pH恒定进料和恒速进料相比，DO恒定进料方法具有最高的肝素滴度，为8.63 g / L，是分批培养的9倍。获得的最佳甘油进料策略可能对扩大微生物肝素生产的规模有用。

著录项

  • 来源
    《Bioprocess and Biosystems Engineering》 |2012年第7期|p.1209-1218|共10页
  • 作者

    Yanfeng Liu; Long Liu; Jinghua Chen; Jianghua Li; Guocheng Du; Jian Chen;

  • 作者单位

    Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China,School of Biotechnology, Jiangnan University, Wuxi 214122, China;

    Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China,School of Biotechnology, Jiangnan University, Wuxi 214122, China;

    School of Medicine and Pharmaceutics, Jiangnan University, Wuxi 214122, China,National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi 214122, China;

    Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China;

    Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China,School of Biotechnology, Jiangnan University, Wuxi 214122, China;

    Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    heparosan; feeding strategy; glycerol; escherichia coli K5;

    机译：肝素喂养策略;甘油大肠杆菌K5;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号