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首页> 外文期刊>Bioprocess and Biosystems Engineering >Construction of an amperometric glucose biosensor based on the immobilization of glucose oxidase onto electrodeposited Pt nanoparticles-chitosan composite film
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Construction of an amperometric glucose biosensor based on the immobilization of glucose oxidase onto electrodeposited Pt nanoparticles-chitosan composite film

机译：基于葡萄糖氧化酶固定在电沉积Pt纳米颗粒-壳聚糖复合膜上的安培型葡萄糖生物传感器的构建

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摘要

One-step construction of Pt nanoparticles-chitosan composite film (PtNPs-CS) was firstly proposed as a novel immobilization matrix for the enzymes to fabricate glucose biosensor. This novel interface embedded in situ PtNPs in CS hydrogel was developed by one-step electrochemical deposition in solution containing CS and chloro-platinic acid (H_2PtCl_6). Several techniques, including scanning electron microscopy (SEM), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and chronoamperometry were employed to characterize the assembly process and performance of the biosensor. Under the optimized experimental conditions, the resulting biosensor exhibited excellent linear behavior in the concentration range from 1.2 μM to 4.0 mM for the quantitative analysis of glucose with a limit of detection of 0.4 μM at a signal-to-noise ratio of 3. The apparent Michaelis-Menten constant (K_m~(app)) was evaluated to be 2.4 mM, showing good affinity. The proposed biosensor offered good amperometric responses to glucose due to the nanostructured sensing film provided plenty of active sites for the immobilization of glucose oxidase (GOD).
机译：首先提出了Pt纳米粒子-壳聚糖复合膜（PtNPs-CS）的一步构建作为酶制备葡萄糖生物传感器的新型固定基质。通过在包含CS和氯铂酸（H_2PtCl_6）的溶液中进行一步电化学沉积，开发出了一种在CS水凝胶中嵌入原位PtNPs的新型界面。包括扫描电子显微镜（SEM），循环伏安法（CV），电化学阻抗谱（EIS）和计时电流法在内的几种技术用于表征生物传感器的组装过程和性能。在优化的实验条件下，所得的生物传感器在1.2μM至4.0 mM的浓度范围内表现出出色的线性行为，用于葡萄糖的定量分析，信噪比为3时检测限为0.4μM。 Michaelis-Menten常数（K_m〜（app））被评估为2.4 mM，显示出良好的亲和力。由于纳米结构的传感膜为葡萄糖氧化酶（GOD）的固定提供了足够的活性位点，因此提出的生物传感器对葡萄糖具有良好的安培响应。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2012年第7期|p.1089-1095|共7页
  • 作者

    Jingjing Li; Ruo Yuan; Yaqin Chai; Xin Che; Wenjuan Li;

  • 作者单位

    Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education and Key Laboratory of Eco-environments in Three Gorges Reservoir Region, College of Chemistry and Chemical Engineering, Southwest University, 400715 Chongqing, People's Republic of China;

    Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education and Key Laboratory of Eco-environments in Three Gorges Reservoir Region, College of Chemistry and Chemical Engineering, Southwest University, 400715 Chongqing, People's Republic of China;

    Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education and Key Laboratory of Eco-environments in Three Gorges Reservoir Region, College of Chemistry and Chemical Engineering, Southwest University, 400715 Chongqing, People's Republic of China;

    Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education and Key Laboratory of Eco-environments in Three Gorges Reservoir Region, College of Chemistry and Chemical Engineering, Southwest University, 400715 Chongqing, People's Republic of China;

    Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education and Key Laboratory of Eco-environments in Three Gorges Reservoir Region, College of Chemistry and Chemical Engineering, Southwest University, 400715 Chongqing, People's Republic of China;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    glucose oxidase; glucose biosensor; one-step electrodeposition; pt nanoparticle; chitosan; composite film;

    机译：葡萄糖氧化酶葡萄糖生物传感器一步电沉积pt纳米粒子壳聚糖复合膜;

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