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首页> 外文期刊>Bioprocess and Biosystems Engineering >Lipase catalyzed acetylation of 3,5,4'-trihydroxystilbene: optimization and kinetics study
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Lipase catalyzed acetylation of 3,5,4'-trihydroxystilbene: optimization and kinetics study

机译：脂肪酶催化3,5,4'-三羟基sti的乙酰化：优化和动力学研究

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摘要

The use of immobilized lipase from Candida antarctica (Novozym® 435) to catalyze acetylation of frans-3,5,4'-trihydroxystilbene was investigated in this study. Response surface methodology and 5-level-4-factor central composite rotatable design were adopted to evaluate the effects of synthesis variables, including reaction time (24-72 h), temperature (25-65 ℃), substrate molar ratio (1:15-1:75), and enzyme amount (600-3,000 PLU) on the percentage molar conversion of trans-4'-O-aeety-3,5-dihydroxystilbene. The results showed that reaction temperature and enzyme amount were the most important parameters on percentage molar conversion. Based on ridge max analysis, the optimum conditions for synthesis were: reaction time 60 h, reaction temperature 64 ℃, substrate molar ratio 1:56 and enzyme amount 2,293 PLU. The molar conversion of actual experimental values was 95% under optimal conditions. The synthesis product was analyzed using HPLC, mass and NMR. The results revealed that the major product was trans-4'-O-acetyl-3,5-dihydroxystilbene. The reaction kinetics was found to follow the Ping-Pong mechanism; substrate inhibition was not found at high vinyl acetate concentration.
机译：在这项研究中，研究了使用固定化的南极假丝酵母脂肪酶（Novozym®435）催化fans-3,5,4'-三羟基sti的乙酰化。采用响应面法和五级四因子中心复合材料可旋转设计评价反应时间（24-72 h），温度（25-65℃），底物摩尔比（1:15）对合成变量的影响。 -1：75）和酶含量（600-3,000 PLU），以反式4'-O-aeety-3,5-二羟基hydroxy的摩尔转化率为基准。结果表明，反应温度和酶用量是影响摩尔转化率最重要的参数。根据岭最大值分析，最佳合成条件为：反应时间60 h，反应温度64℃，底物摩尔比1:56和酶量2,293 PLU。在最佳条件下，实际实验值的摩尔转化率为95％。使用HPLC，质量和NMR分析合成产物。结果表明，主要产物为反式-4'-O-乙酰基-3,5-二羟基sti。发现反应动力学遵循乒乓机制。在高乙酸乙烯酯浓度下未发现底物抑制。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2012年第7期|p.1137-1145|共9页
  • 作者

    Chia-Hung Kuo; Fang-Wen Hsiao; Shu-Mei Dai; Chieh-Ming J. Chang; Chih-Chen Lee; Yung-Chuan Liu; Chwen-Jen Shieh;

  • 作者单位

    Biotechnology Center, National Chung Hsing University, 250 Kuo-kuang Road, Taichung 402, Taiwan;

    Department of Chemical Engineering, National Chung Hsing University, 250 Kuo-Kuang Road, Taichung 402, Taiwan;

    Department of Entomology, National Chung Hsing University, 250 Kuo-Kuang Road, Taichung 402, Taiwan;

    Department of Chemical Engineering, National Chung Hsing University, 250 Kuo-Kuang Road, Taichung 402, Taiwan;

    Department of Chemical Engineering, National Chung Hsing University, 250 Kuo-Kuang Road, Taichung 402, Taiwan;

    Department of Chemical Engineering, National Chung Hsing University, 250 Kuo-Kuang Road, Taichung 402, Taiwan;

    Biotechnology Center, National Chung Hsing University, 250 Kuo-kuang Road, Taichung 402, Taiwan;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    lipase acetylation; hydroxystilbene; optimization; kinetics; response surface methodology;

    机译：脂肪酶乙酰化;羟基二苯乙烯;优化;动力学;响应面法;

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