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首页> 外文期刊>Bioprocess and Biosystems Engineering >Substrate mass transport in two-phase partitioning bioreactors employing liquid and solid non-aqueous phases
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Substrate mass transport in two-phase partitioning bioreactors employing liquid and solid non-aqueous phases

机译：使用液相和固体非水相的两相分配式生物反应器中的基质传质

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摘要

The mass transfer of phenol and butyl acetate to/from water was studied in two-phase partitioning bioreactors using immiscible organic solvents and solid polymer beads as the partitioning phases in a 5-L stirred tank bioreactor. Virtually instantaneous mass transfer was observed with phenol in water/2-undecanone, and with butyl acetate in water/silicone oil systems. The mass transfer of butyl acetate to silicone oil was rapid irrespective of the viscosity of the partitioning phase. When Hytrel® polymer beads were employed as the partitioning phase, substrate transport to the polymer was found not to be externally mass transfer limited, but rather internally by substrate diffusion into the polymer. In contrast to gaseous, poorly soluble substrates studied in other works, mass transfer of soluble substrates such as phenol and butyl acetate to the polymer was unaffected by impeller speed but rather by polymer mass fraction.
机译：在两相分配生物反应器中研究了苯酚和乙酸丁酯与水之间的传质，其中使用了不混溶的有机溶剂和固体聚合物珠作为5 L搅拌釜生物反应器中的分配相。在水/ 2-十一烷酮中用苯酚，在水/硅油体系中用乙酸丁酯观察到几乎瞬时的传质。不管分配相的粘度如何，乙酸丁酯向硅油的质量转移都是快速的。当将Hytrel®聚合物珠粒用作分隔相时，发现向聚合物的底物传输不受外部传质限制，而是内部受底物扩散到聚合物中的传递。与在其他工作中研究的气态，难溶性底物相反，可溶性底物（例如苯酚和乙酸丁酯）向聚合物的传质不受叶轮速度的影响，而受聚合物质量分数的影响。

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