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首页> 外文期刊>Bioprocess and Biosystems Engineering >Cross-linking 01 horseradish peroxidase adsorbed on polycatiomc films: utilization for direct dye degradation
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Cross-linking 01 horseradish peroxidase adsorbed on polycatiomc films: utilization for direct dye degradation

机译：交联的01辣根过氧化物酶吸附在polycatiomc膜上：用于直接染料降解

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摘要

Horseradish peroxidase (HRP) was immobilized on the polyaniline (PANI) grafted polyacrylonitrile (PAN) films. The maximum HRP immobilization capacity of the PAN-g-PANI-3 film was 221 ug/cm~2. The HRP-immobilized PAN-g-PANI-3 film retained 79 % of the activity of the same quantity free enzyme. The HRP-immobilized PAN-g-PANI-3 film was operated for the decolorization of two different benzidine-based dyes in the presence of hydrogen peroxide. The maximum decolorization grade was obtained at pH 6.0 for both dyes. The HRP-immobilized PAN-g-PANI-3 film was very effective for removal of Direct Blue-53 compared to Direct Black-38 from aqueous solutions. The immobilized HRP exhibited high resistance to proteolysis by trypsin compared to the free counterpart. Immobilized HRP preserved 83 % of its original activity even after 8 weeks of storage at 4 ℃, while the free enzyme lost its initial activity after 3 weeks of storage period.
机译：将辣根过氧化物酶（HRP）固定在聚苯胺（PANI）接枝的聚丙烯腈（PAN）膜上。 PAN-g-PANI-3膜的最大HRP固定容量为221 ug / cm〜2。固定有HRP的PAN-g-PANI-3膜保留了相同数量游离酶的79％的活性。在过氧化氢的存在下，将固定有HRP的PAN-g-PANI-3膜用于两种不同的基于联苯胺的染料的脱色。两种染料的最大脱色等级均为pH 6.0。与从水溶液中直接制黑38相比，固定有HRP的PAN-g-PANI-3膜对直接制蓝53的去除非常有效。固定的HRP与游离的HRP相比，对胰蛋白酶的蛋白水解表现出较高的抵抗力。固定化的HRP甚至在4℃下保存8周后仍能保留其原始活性的83％，而游离酶在保存3周后仍失去了其初始活性。

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