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首页> 外文期刊>Bioprocess and Biosystems Engineering >Optimization of process parameters by response surface methodology and kinetic modeling for batch production of canthaxanthin by Dietzia maris NIT-D (accession number: HM151403)
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Optimization of process parameters by response surface methodology and kinetic modeling for batch production of canthaxanthin by Dietzia maris NIT-D (accession number: HM151403)

机译：通过响应面方法和动力学建模优化工艺参数，以用于由Dietzia maris NIT-D批量生产角黄素（登录号：HM151403）

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摘要

Dietzia maris NIT-D, a canthaxanthin producer, was isolated during routine screening of pigment-producing bacteria. Response surface methodology was applied for statistical designing of process parameters for biomass and canthaxanthin production. The effects of four process parameters (considered as independent variables), namely temperature (10-30 ℃), pH (4.75-5.75), shaker speed (75-135 rpm) and percentage inoculum (0.5-2.5 %) on the biomass and canthaxanthin yield (considered as dependent variables) were studied. As much as 122 mg L~(-1) of canthaxanthin was obtained when Dietzia maris NIT-D was incubated for 120 h at 25 ℃ and 120 rpm, initial pH and percentage inoculum being 5.5 and 2 % respectively. The pigment yield is the highest reported till date, with Dietzia maris as the test organism. The maximum biomass yield was 7.39 g L~(-1) under optimized process parameters. The predicted values were also verified by validation experiments in 5-day fermentation. Different mathematical models were used to describe growth and production, considering the effect of glucose in batch mode. The kinetic constants were calculated by fitting the experimental data to the models. Cell growth was inhibited beyond a glucose concentration of 15 g L~'. Andrews' model gave the best fit with a R2 value of 0.9993. During the exponential growth phase, the specific growth rate was found to remain fairly constant with respect to time. There was no inhibitory effect due to intracellular product accumulation for all concentrations of glucose. This observation is the first of its kind, as previous studies have reported that increasing accumulation of intracellular carotenoid exerts greater degree of inhibition on growth.
机译：在常规筛选产色素细菌的过程中，分离出了黄tha素生产者Dietzia maris NIT-D。将响应面方法应用于生物质和角黄素生产过程参数的统计设计。温度（10-30℃），pH（4.75-5.75），振动筛速度（75-135 rpm）和接种量百分比（0.5-2.5％）这四个工艺参数对生物量的影响研究了角黄素的产量（被认为是因变量）。 Dietzia maris NIT-D在25℃和120 rpm孵育120 h时可得到高达122 mg L〜（-1）的角黄素，初始pH值和接种量分别为5.5和2％。迄今为止，色素产量是迄今为止报道的最高水平，其中马氏Diet为测试生物。在优化工艺参数下，最大生物量为7.39 g L〜（-1）。还通过5天发酵中的验证实验来验证预测值。考虑到分批模式下葡萄糖的影响，使用了不同的数学模型来描述生长和生产。通过将实验数据拟合到模型中来计算动力学常数。超过15 g L''的葡萄糖浓度，细胞生长受到抑制。安德鲁斯的模型以0.9993的R2值给出了最佳拟合。在指数增长阶段，发现特定的增长率相对于时间保持相当恒定。对于所有浓度的葡萄糖，由于细胞内产物的积累而没有抑制作用。这种观察是第一个此类观察，因为以前的研究已经报道，细胞内类胡萝卜素积累的增加对生长具有更大程度的抑制作用。

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