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首页> 外文期刊>Bioprocess and Biosystems Engineering >Effect of culture conditions on synthesis of triterpenoids in suspension cultures of Lantana camara L.
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Effect of culture conditions on synthesis of triterpenoids in suspension cultures of Lantana camara L.

机译：培养条件对马tana丹悬浮培养中三萜类化合物合成的影响

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Present report is aimed to study the batch kinetics of Lantana camara. Dynamic changes of parameters, such as pH, conductivity, wet and dry cell concentrations, consumption of major nutrients, carbon source and agitation speeds were investigated to understand the culture characteristics of suspended cells grown on MS + BAP + 2,4-D + NAA in shake flasks. Results indicated that the consumption of phosphate resulted in the onset of stationary phase in cultures. Maltose as carbon source resulted in production of maximum triterpenoid content (31.08 mg/L) while the least was found on glucose (10.69 mg/L). Notably, both did not support accumulation of betulinic acid. Sucrose, although stood second in terms of quantity (21.6 mg/L), supported the production of all the three triterpenoids-oleanolic, ursolic and betulinic acids. Maximum viable cultures were obtained at a rotation speed of 120 rpm. The present finding will form a background for further scale-up related studies.
机译：本报告旨在研究马tana丹（Lantana camara）的批动力学。研究了pH，电导率，干细胞和干细胞的浓度，主要营养物质的消耗，碳源和搅拌速度等参数的动态变化，以了解在MS + BAP + 2,4-D + NAA上生长的悬浮细胞的培养特性在摇瓶中。结果表明，磷酸盐的消耗导致培养物中出现固定相。麦芽糖作为碳源导致最大的三萜含量（31.08 mg / L），而葡萄糖含量最低（10.69 mg / L）。值得注意的是，两者均不支持桦木酸的积累。蔗糖虽然以数量计（21.6 mg / L）排在第二位，但它支持所有三种三萜类油酸，熊果酸和桦木酸的生产。在120 rpm的转速下获得最大可行的培养物。本发现将为进一步扩大相关研究提供背景。

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