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首页> 外文期刊>Bioprocess and Biosystems Engineering >Heterotrophic growth and lipid production of Chlorella protothecoides on glycerol
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Heterotrophic growth and lipid production of Chlorella protothecoides on glycerol

机译：原绿小球藻在甘油上的异养生长和脂质产生

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During the production of biodiesel, a significant amount of glycerol is generated which currently has little commercial value. A study on the growth and lipid production of Chlorella protothecoides using glycerol as the carbon source was performed to demonstrate the utility of recycling crude glycerol created during biodiesel production. Glycerol was examined as both the sole carbon source and in combination with glucose. Algae cultures grown on only glycerol in shake flasks showed a specific growth rate and final lipid yield of 0.1/h and 0.31 g lipid/g substrate, respectively. The values were similar to those observed on pure glucose, 0.096/h and 0.24 g lipid/g substrate. When the media contained a mixture of glycerol and glucose, simultaneous uptake of the two substrates was observed. Due to the difference in rates of lipid storage, lipid production was 0,077 g lipid/(1 h) during growth on glycerol, while growth on glucose had a productivity of 0.096 g lipid/(1 h). During growth on the 9:1 mixture of both glucose and glycerol, lipid productivity was 0.098 g lipid/(1 h). In order to simulate the use of waste glycerol from biodiesel production the experiments were repeated and similar growth rates, yields, and lipid productivities were achieved. Therefore, we have demonstrated the promise for simultaneous high growth rates and lipid yields of C. protothecoides heterotrophically grown on mixtures of glycerol.
机译：在生物柴油的生产过程中，产生了大量的甘油，目前其商业价值很小。进行了使用甘油作为碳源的原球藻小球藻生长和脂质生产的研究，以证明回收利用在生物柴油生产过程中产生的粗甘油的效用。甘油是唯一的碳源，而且与葡萄糖结合使用。仅在摇瓶中的甘油上生长的藻类培养物显示出特定的生长速率，最终脂质产量分别为0.1 / h和0.31 g脂质/ g底物。该值类似于在纯葡萄糖，0.096 / h和0.24 g脂质/ g底物上观察到的值。当培养基含有甘油和葡萄糖的混合物时，观察到同时吸收了两种底物。由于脂质存储速率的差异，在甘油上生长期间脂质产量为0,077 g脂质/（1 h），而在葡萄糖上生长的生产力为0.096 g脂质/（1 h）。在葡萄糖和甘油的9：1混合物上生长期间，脂质生产力为0.098 g脂质/（1 h）。为了模拟生物柴油生产中废甘油的使用，重复了实验，并获得了相似的生长速率，产量和脂质生产率。因此，我们证明了在甘油的混合物上异养生长的原毛球藻同时具有较高的生长速率和脂质产量。

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