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首页> 外文期刊>Bioprocess and Biosystems Engineering >Influence of drying methods over in vitro antitumoral effects of exopolysaccharides produced by Agaricus blazei LPB 03 on submerged fermentation
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Influence of drying methods over in vitro antitumoral effects of exopolysaccharides produced by Agaricus blazei LPB 03 on submerged fermentation

机译：干燥方法对姬松茸LPB 03产生的胞外多糖体外发酵抗肿瘤作用的影响

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摘要

Agaricus blazei is a mushroom that belongs to the Brazilian biodiversity and is considered as an important producer of bioactive compounds beneficial to human health. Studies have demonstrated that these compounds present immuno-modulatory, antioxidant and antitumor properties. In order to compare the most used method for fungal polysaccharide drying, lyophilization with other industrial-scale methods, the aim of this work was to submit A. blazei LPB 03 polysaccharide extracts to vaucum, spray and freeze drying, and evaluate the maintenance of its antitumoral effects in vitro. Exopolysaccharides produced by A. blazei LPB 03 on submerged fermentation were extracted with ethanol and submitted to drying processes. The efficiency represents the water content that was removed during the drying process. The resultant dried products showed water content around 3% and water activity less than 0.380, preventing therefore the growth of microorganisms and reactions of chemical degradation. Exopolysaccharide extracts dried by vacuum and spray dryer did not showed any significant cytotoxic effect on cell viability of Wistar mice macrophages. Content of total sugars and protein decrease after drying, nevertheless, 20 mg/ml of exopolysaccharides dried by spray dryer reached 33% of inhibition rate over Ehrlich tumor cells in vitro.
机译：姬松茸是属于巴西生物多样性的蘑菇，被认为是有益于人类健康的重要生物活性化合物的生产者。研究表明，这些化合物具有免疫调节，抗氧化和抗肿瘤特性。为了将最常用的真菌多糖干燥，冻干方法与其他工业规模方法进行比较，这项工作的目的是将A. blazei LPB 03多糖提取物进行真空，喷雾和冷冻干燥，并评估其维持性。体外抗肿瘤作用。用乙醇提取由A. blazei LPB 03进行的深层发酵产生的胞外多糖，并进行干燥处理。效率表示在干燥过程中除去的水分。所得干燥产物显示出约3％的水含量和小于0.380的水活度，因此防止了微生物的生长和化学降解的反应。通过真空和喷雾干燥器干燥的胞外多糖提取物对Wistar小鼠巨噬细胞的细胞活力没有显示任何明显的细胞毒性作用。干燥后总糖和蛋白质含量降低，然而，通过喷雾干燥器干燥的20mg / ml的胞外多糖在体外对Ehrlich肿瘤细胞达到了33％的抑制率。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2011年第3期|p.253-261|共9页
  • 作者

    M. B. A. Fcrnandes; S. Habu; M. A. de Lima; V. Thomaz-Soccol; C. R. Soccol;

  • 作者单位

    Bioprocess Engineering and Biotechnology Division, Federal University of Parana, UFPR, Post Office Box 19011, Curitiba, PR 81531-990, Brazil;

    Bioprocess Engineering and Biotechnology Division, Federal University of Parana, UFPR, Post Office Box 19011, Curitiba, PR 81531-990, Brazil;

    Bioprocess Engineering and Biotechnology Division, Federal University of Parana, UFPR, Post Office Box 19011, Curitiba, PR 81531-990, Brazil;

    Bioprocess Engineering and Biotechnology Division, Federal University of Parana, UFPR, Post Office Box 19011, Curitiba, PR 81531-990, Brazil;

    Bioprocess Engineering and Biotechnology Division, Federal University of Parana, UFPR, Post Office Box 19011, Curitiba, PR 81531-990, Brazil;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    agaricus blazei; macrophages cytotoxicity; submerged fermentation; exopolysaccharides production; spray drying; antitumoral effect;

    机译：姬松茸巨噬细胞的细胞毒性;水下发酵胞外多糖的产生;喷雾干燥抗肿瘤作用;

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