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首页> 外文期刊>Bioprocess and Biosystems Engineering >Single step concomitant concentration, purification and characterization of two families of lipopeptides of marine origin
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Single step concomitant concentration, purification and characterization of two families of lipopeptides of marine origin

机译：一步一步同时浓缩，纯化和鉴定两个海洋脂肽家族

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摘要

The extracellular biosurfactant product secreted by a marine bacterium was concentrated and purified directly from the fermentation broth in a single step by ultrafiltration (UF) employing YM 30 kDa (UF-I) and Omega 10 kDa (UF-II) polyethersulfone membranes. The optimum operating pressure required for both membranes, UF-I and UF-II, were found to be 30 and 35 psi, respectively. The biosurfactant from the fermentation broth was recovered in higher amounts using UF-II (89%) than using UF-I (73%). An analysis of the critical micelle concentrations (CMC) of the recovered lipopeptides showed a lower CMC value of 15 mg L~(-1) for the UF-II product, indicating higher degree of purity (83%) when compared to that of the UF-I product (78%). The ultrafiltered products were characterized using Fourier transformed infrared spectroscopy and matrix-assisted laser desorption ioniza-tion time of flight mass spectral analysis, which demonstrated the presence of two families of lipopeptides.
机译：通过使用YM 30 kDa（UF-I）和Omega 10 kDa（UF-II）聚醚砜膜的超滤（UF），一步一步浓缩和纯化由海洋细菌分泌的细胞外生物表面活性剂产品，直接从发酵液中纯化。两种膜UF-I和UF-II所需的最佳操作压力分别为30 psi和35 psi。使用UF-II（89％）比使用UF-I（73％）从发酵液中回收的生物表面活性剂含量更高。对回收的脂肽的临界胶束浓度（CMC）的分析显示，UF-II产品的CMC值较低，为15 mg L〜（-1），表明与纯品相比，其较高的纯度（83％） UF-1产品（78％）。使用傅里叶变换红外光谱和基质辅助激光解吸电离飞行时间质谱分析法对超滤产物进行了表征，证明了存在两个脂肽家族。

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