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Sulfuric acid pretreatment and enzymatic hydrolysis of photoperiod sensitive sorghum for ethanol production

机译：光周期敏感高粱的硫酸预处理和酶促水解生产乙醇

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摘要

Photoperiod sensitive (PS) sorghum, with high soluble sugar content, high mass yield and high drought tolerance in dryland environments, has great potential for bioethanol production. The effect of diluted sulfuric acid pretreatment on enzymatic hydrolysis was investigated. Hydrolysis efficiency increased from 78.9 to 94.4% as the acid concentration increased from 0.5 to 1.5%. However, the highest total glucose yield (80.3%) occurred at the 1.0% acid condition because of the significant cellulose degradation at the 1.5% concentration. Synchrotron wide-angle X-ray diffraction was used to study changes of the degree of crystallinity. With comparison of cellulosic crystallinity and adjusted cellulosic crystallinity, the crystalline cellulose decreased after low acidic concentration (0.5%) applied, but did not change significantly, as the acid concentration increased. Scanning electron microscopy was also employed to understand how the morphological structure of PS sorghum changed after pretreatment. Under current processing conditions, the total ethanol yield is 74.5% (about 0.2 g ethanol from 1 g PS sorghum). A detail mass balance was also provided.
机译：对光周期敏感（PS）的高粱，在干旱地区环境中具有高可溶性糖含量，高产量和高抗旱性，在生物乙醇生产方面具有巨大潜力。研究了稀硫酸预处理对酶促水解的影响。随着酸浓度从0.5％增加到1.5％，水解效率从78.9％增加到94.4％。但是，由于在1.5％的浓度下纤维素会发生明显降解，因此在1.0％的酸性条件下会出现最高的总葡萄糖产量（80.3％）。用同步加速器广角X射线衍射研究结晶度的变化。通过比较纤维素结晶度和调节的纤维素结晶度，在施加低酸浓度（0.5％）后结晶纤维素减少，但随着酸浓度增加，结晶纤维素没有明显变化。扫描电子显微镜还用于了解预处理后PS高粱的形态结构如何变化。在当前的加工条件下，乙醇的总产率为74.5％（1 g PS高粱中约有0.2 g乙醇）。还提供了详细的质量平衡。

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