...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Bioprocess and Biosystems Engineering >Multi-stage high cell continuous fermentation for high productivity and titer
【24h】

Multi-stage high cell continuous fermentation for high productivity and titer

机译：多阶段高细胞连续发酵可实现高生产率和滴定度

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We carried out the first simulation on multistage continuous high cell density culture (MSC-HCDC) to show that the MSC-HCDC can achieve batch/fed-batch product titer with much higher productivity to the fed-batch productivity using published fermentation kinetics of lactic acid, penicillin and ethanol. The system under consideration consists of n-serially connected continuous stirred-tank reactors (CSTRs) with either hollow fiber cell recycling or cell immobilization for high cell-density culture. In each CSTR substrate supply and product removal are possible. Penicillin production is severely limited by glucose metabolite repression that requires multi-CSTR glucose feeding. An 8-stage C-HCDC lactic acid fermentation resulted in 212.9 g/L of titer and 10.6 g/L/h of productivity, corresponding to 101 and 429% of the comparable lactic acid fed-batch, respectively. The penicillin production model predicted 149% (0.085 g/L/h) of productivity in 8-stage C-HCDC with 40 g/L of cell density and 289% of productivity (0.165 g/L/h) in 7-stage C-HCDC with 60 g/L of cell density compared with referring batch cultivations. A 2-stage C-HCDC ethanol experimental run showed 107% titer and 257% productivity of the batch system having 88.8 g/L of titer and 3.7 g/L/h of productivity. MSC-HCDC can give much higher productivity than batch/fed-batch system, and yield a several percentage higher titer as well. The productivity ratio of MSC-HCDC over batch/fed-batch system is given as a multiplication of system dilution rate of MSC-HCDC and cycle time of batch/fed-batch system. We suggest MSC-HCDC as a new production platform for various fermentation products including monoclonal antibody.
机译：我们对多阶段连续高细胞密度培养（MSC-HCDC）进行了首次模拟，结果表明MSC-HCDC可以利用已公布的乳酸发酵动力学，以分批/补料分批的产品效价比补料分批的生产率高得多。酸，青霉素和乙醇。所考虑的系统由n串行连接的连续搅拌釜反应器（CSTR）和中空纤维细胞回收或高细胞密度培养的细胞固定化组成。在每个CSTR基板中，都可以供应和移除产品。青霉素的生产受到葡萄糖代谢物抑制的严重限制，葡萄糖代谢物抑制需要多CSTR葡萄糖喂养。 8阶段C-HCDC乳酸发酵可产生212.9 g / L的滴度和10.6 g / L / h的生产率，分别相当于可比较的乳酸补料分批的101％和429％。青霉素生产模型预测8级C-HCDC的生产率为149％（0.085 g / L / h），其中细胞密度为40 g / L，7级C的产率为289％（0.165 g / L / h）与参考分批培养相比，具有60 g / L细胞密度的-HCDC。两阶段C-HCDC乙醇实验运行显示，滴定度为88.8 g / L，生产率为3.7 g / L / h的批处理系统，其滴定度为107％，产率为257％。 MSC-HCDC的生产率比分批/补料分批系统高得多，滴度也高出几个百分点。 MSC-HCDC在分批/补料分批系统上的生产率比为MSC-HCDC的系统稀释率与分批/补料分批系统的循环时间的乘积。我们建议将MSC-HCDC作为各种发酵产品（包括单克隆抗体）的新生产平台。

著录项

  • 来源
    《Bioprocess and Biosystems Engineering》 |2011年第4期|p.419-431|共13页
  • 作者

    Ho Nam Chang; Nag-Jong Kim; Jongwon Kang; Chang Moon Jeong; Jin-dal-rae Choi; Qiang Fei; Byoung Jin Kim; Sunhoon Kwon; Sang Yup Lee; Jungbae Kim;

  • 作者单位

    Department of Chemical and Biomolecular Engineering,KAIST (Korea Advanced Institute of Science and Technology),Daejeon 305-701, Korea;

    Department of Chemical and Biomolecular Engineering,KAIST (Korea Advanced Institute of Science and Technology),Daejeon 305-701, Korea,Samsung Petrochemical, Yongin-si 446-712, Korea;

    Department of Chemical and Biomolecular Engineering,KAIST (Korea Advanced Institute of Science and Technology),Daejeon 305-701, Korea;

    Department of Chemical and Biomolecular Engineering,KAIST (Korea Advanced Institute of Science and Technology),Daejeon 305-701, Korea;

    Department of Chemical and Biomolecular Engineering,KAIST (Korea Advanced Institute of Science and Technology),Daejeon 305-701, Korea;

    Department of Chemical and Biomolecular Engineering,KAIST (Korea Advanced Institute of Science and Technology),Daejeon 305-701, Korea;

    Department of Chemical and Biomolecular Engineering,KAIST (Korea Advanced Institute of Science and Technology),Daejeon 305-701, Korea,Samsung Advanced Institute of Technology,Yongin-si 446-712, Korea;

    Department of Chemical and Biomolecular Engineering,KAIST (Korea Advanced Institute of Science and Technology),Daejeon 305-701, Korea,BINEX, LLC, Seoul 121-815, Korea;

    Department of Chemical and Biomolecular Engineering,KAIST (Korea Advanced Institute of Science and Technology),Daejeon 305-701, Korea;

    Department of Chemical and Biological Engineering,Korea University, Seoul 136-701, Korea;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    multi-stage continuous fermentation; high cell density; ethanol; lactic acid; antibody;

    机译：多阶段连续发酵高细胞密度乙醇乳酸抗体;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号