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A new atomic absorption spectral assay for the determination of trace IgG using immunonanogold

机译：一种使用免疫纳米金测定痕量IgG的新原子吸收光谱法

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摘要

Nanogold in size of 10 nm was used to label goat anti-human IgG (GIgG) to obtain an immunonanogold probe (AuGIgG) for IgG. In pH 6.8 phosphate buffer solution and in the presence of immunoprecipitator polyethylene glycol 6000 (PEG 6000), IgG reacted with the probe (AuGIgG) to form AuGIgG-IgG-PEG immuno-complex. After the centrifugation to remove the immuno-complex, AuGIgG in the supernatant can be measured by atomic absorption spectrophotometry at gold absorption line 242.8 nm. The results showed that the absorption value decreased as the concentration of IgG increased, and the decreased absorption value was linear to IgG concentration in the range 0.025-0.375 μg/mL, with a detection limit of 0.008 μg/mL. On this base, a new nanogold-labeled atomic absorption spectral assay for IgG was established. The assay was applied to determine IgG in human serum sample with satisfactory results.
机译：使用大小为10 nm的纳米金标记山羊抗人IgG（GIgG），以获得IgG的免疫纳米金探针（AuGIgG）。在pH 6.8磷酸盐缓冲溶液中，在存在免疫沉淀器聚乙二醇6000（PEG 6000）的情况下，IgG与探针（AuGIgG）反应形成AuGIgG-IgG-PEG免疫复合物。离心去除免疫复合物后，可以通过原子吸收分光光度法在金吸收线242.8 nm处测量上清液中的AuGIgG。结果表明，随着IgG浓度的增加，吸收值降低，吸收值的降低与IgG浓度在0.025-0.375μg/ mL范围内呈线性关系，检出限为0.008μg/ mL。在此基础上，建立了一种新的纳米金标记的IgG原子吸收光谱测定法。该测定法用于测定人血清样品中的IgG，结果令人满意。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2011年第4期|p.471-476|共6页
  • 作者

    Yafang Tang; Caina Jiang; Aihui Liang; Jishun Li; Zhiliang Jiang;

  • 作者单位

    Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education,School of Environment and Resource,Guangxi Normal University, Guilin 541004, China;

    Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education,School of Environment and Resource,Guangxi Normal University, Guilin 541004, China;

    Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education,School of Environment and Resource,Guangxi Normal University, Guilin 541004, China;

    Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education,School of Environment and Resource,Guangxi Normal University, Guilin 541004, China;

    Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education,School of Environment and Resource,Guangxi Normal University, Guilin 541004, China;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    IgG; immunonanogold; atomic absorption spectrometry;

    机译：IgG;免疫纳米金原子吸收光谱法;

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