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首页> 外文期刊>Bioprocess and Biosystems Engineering >Enzymatic synthesis of farnesyl laurate in organic solvent: initial water activity, kinetics mechanism, optimization of continuous operation using packed bed reactor and mass transfer studies
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Enzymatic synthesis of farnesyl laurate in organic solvent: initial water activity, kinetics mechanism, optimization of continuous operation using packed bed reactor and mass transfer studies

机译：在有机溶剂中酶法合成月桂酸法呢酯：初始水活度，动力学机理，使用填充床反应器的连续操作优化和传质研究

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摘要

The influence of water activity and water content was investigated with farnesyl laurate synthesis catalyzed by Lipozyme RM IM. Lipozyme RM IM activity depended strongly on initial water activity value. The best results were achieved for a reaction medium with an initial water activity of 0.11 since it gives the best conversion value of 96.80%. The rate constants obtained in the kinetics study using Ping-Pong-Bi-Bi and Ordered-Bi-Bi mechanisms with dead-end complex inhibition of lauric acid were compared. The corresponding parameters were found to obey the Ordered-Bi-Bi mechanism with dead-end complex inhibition of lauric acid. Kinetic parameters were calculated based on this model as follows: V_(max) = 5.80 mmol I~(-1) min~(-1) g enzyme~(-1), K_(m,A) = 0.70 mmol I~(-1) g enzyme~(-1), K_(m,B) = 115.48 mmol I~(-1) g enzyme~(-1), K_i = 11.25 mmol I~(-1) g enzyme~(-1). The optimum conditions for the esterification of farnesol with lauric acid in a continuous packed bed reactor were found as the following: 18.18 cm packed bed height and 0.9 ml/min substrate flow rate. The optimum molar conversion of lauric acid to farnesyl laurate was 98.07±0.82%. The effect of mass transfer in the packed bed reactor has also been studied using two models for cases of reaction limited and mass transfer limited. A very good agreement between the mass transfer limited model and the experimental data obtained indicating that the esterification in a packed bed reactor was mass transfer limited.
机译：用Lipozyme RM IM催化的月桂酸法呢酯合成研究了水活度和水含量的影响。 Lipozyme RM IM活性很大程度上取决于初始水分活度值。对于初始水活度为0.11的反应介质，获得了最佳结果，因为它具有96.80％的最佳转化率。比较了在动力学研究中使用Ping-Pong-Bi-Bi和有序的Bi-Bi机理对月桂酸有最终抑制作用的速率常数。发现相应的参数服从有序的Bi-Bi机制，具有月桂酸的末端末端复合物抑制作用。根据该模型计算动力学参数，如下：V_（max）= 5.80 mmol I〜（-1）min〜（-1）g酶〜（-1），K_（m，A）= 0.70 mmol I〜（ -1）g酶〜（-1），K_（m，B）= 115.48 mmol I〜（-1）g酶〜（-1），K_i = 11.25 mmol I〜（-1）g酶〜（-1 ）。发现在连续填充床反应器中法尼醇与月桂酸酯化的最佳条件如下：填充床高度为18.18 cm，底物流速为0.9 ml / min。月桂酸向月桂酸法呢酯的最佳摩尔转化率为98.07±0.82％。对于反应受限和传质受限的情况，还使用两种模型研究了填充床反应器中的传质效果。传质受限模型与获得的实验数据之间的很好的一致性表明填充床反应器中的酯化是传质受限的。

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