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首页> 外文期刊>Bioprocess and Biosystems Engineering >Immobilization of lipase from Candida rugosa on Sepabeads~®:the effect of lipase oxidation by periodates
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Immobilization of lipase from Candida rugosa on Sepabeads~®:the effect of lipase oxidation by periodates

机译：Sepabeads〜®上固定皱纹念珠菌脂肪酶的作用：高碘酸盐对脂肪酶氧化的影响

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摘要

The objective of this paper was the investigation of a suitable Sepabeads~® support and method for immobilization of lipase from Candida rugosa. Three different supports were used, two with amino groups, (Sepabeads~® EC-EA and Sepabeads~® EC-HA), differing in spacer length (two and six carbons, respectively) and one with epoxy group (Sepabeads~® EC-EP). Lipase immobilization was carried out by two conventional methods (via epoxy groups and via glutaraldehyde), and with periodate method for modification of lipase. The results of activity assays showed that lipase retained 94.8% or 87.6% of activity after immobilization via epoxy groups or with periodate method, respectively, while glutaraldehyde method was inferior with only 12.7% of retention. The immobilization of lipase, previously modified by periodate oxidation, via amino groups has proven to be more efficient than direct immobilization of lipase via epoxy groups. In such a way immobilized enzyme exhibited higher activity at high reaction temperatures and higher thermal stability.
机译：本文的目的是研究合适的Sepabeads®载体和固定皱纹假丝酵母脂肪酶的方法。使用了三种不同的载体，其中两种带有氨基（Sepabeads®EC-EA和Sepabeads®EC-HA），间隔长度不同（分别为两个和六个碳），一个带有环氧基（Sepabeads〜®EC- EP）。脂肪酶的固定是通过两种常规方法（通过环氧基和戊二醛）和高碘酸盐方法进行的。活性测定的结果表明，通过环氧基或高碘酸盐固定化后，脂肪酶保留了94.8％或87.6％的活性，而戊二醛法的保留率仅次于12.7％。已经证明，事先经高碘酸盐氧化修饰的脂肪酶经氨基的固定比通过环氧基的脂肪酶的直接固定更有效。以这种方式，固定化的酶在高反应温度和较高的热稳定性下表现出较高的活性。

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