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Starch hydrolysis modeling: application to fuel ethanol production

机译：淀粉水解建模：在燃料乙醇生产中的应用

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摘要

Efficiency of the starch hydrolysis in the dry grind corn process is a determining factor for overall conversion of starch to ethanol. A model, based on a molecular approach, was developed to simulate structure and hydrolysis of starch. Starch structure was modeled based on a cluster model of amylopectin. Enzymatic hydrolysis of amylose and amylopectin was modeled using a Monte Carlo simulation method. The model included the effects of process variables such as temperature, pH, enzyme activity and enzyme dose. Pure starches from wet milled waxy and high-amylose corn hybrids and ground yellow dent corn were hydrolyzed to validate the model. Standard deviations in the model predictions for glucose concentration and DE values after saccharification were less than ±0.15% (w/v) and ±0.35%, respectively. Correlation coefficients for model predictions and experimental values were 0.60 and 0.91 for liquefaction and 0.84 and 0.71 for saccharification of amylose and amylopectin, respectively. Model predictions for glucose (R~2 = 0.69-0.79) and DP4~+ (R~2 = 0.8-0.68) were more accurate than the maltotriose and maltose for hydrolysis of high-amylose and waxy corn starch. For yellow dent corn, simulation predictions for glucose were accurate (R~2 > 0.73) indicating that the model can be used to predict the glucose concentrations during starch hydrolysis.
机译：干磨玉米过程中淀粉水解的效率是淀粉整体转化为乙醇的决定因素。建立了基于分子方法的模型来模拟淀粉的结构和水解。基于支链淀粉的簇模型，对淀粉结构进行建模。使用蒙特卡罗模拟方法对直链淀粉和支链淀粉的酶水解进行建模。该模型包括过程变量的影响，例如温度，pH，酶活性和酶剂量。将来自湿磨的蜡质和高直链玉米杂交种以及磨碎的黄色齿状玉米的纯淀粉水解以验证模型。糖化后模型预测的葡萄糖浓度和DE值的标准偏差分别小于±0.15％（w / v）和±0.35％。用于模型预测的相关系数和用于液化的实验值的相关系数分别为0.60和0.91，而直链淀粉和支链淀粉的糖化的相关系数分别为0.84和0.71。葡萄糖（R〜2 = 0.69-0.79）和DP4〜+（R〜2 = 0.8-0.68）的模型预测比麦芽三糖和麦芽糖对高直链淀粉和糯玉米淀粉的水解更为准确。对于黄齿玉米，葡萄糖的模拟预测是准确的（R〜2> 0.73），表明该模型可用于预测淀粉水解过程中的葡萄糖浓度。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2011年第7期|p.879-890|共12页
  • 作者

    Ganti S. Murthy; David B. Johnston; Kent D. Rausch; M. E. Tumbleson; Vijay Singh;

  • 作者单位

    Biological and Ecological Engineering, Oregon State University,122 Gilmore Hall, Corvallis, OR 97331, USA,Department of Agricultural and Biological Engineering,University of Illinois, 360G AESB, 1304 West Pennsylvania Avenue, Urbana, IL 61801, USA;

    Eastern Regional Research Center, ARS, USDA, Wyndmoor, PA19038, USA;

    Department of Agricultural and Biological Engineering,University of Illinois, 360G AESB, 1304 West Pennsylvania Avenue, Urbana, IL 61801, USA;

    Department of Agricultural and Biological Engineering,University of Illinois, 360G AESB, 1304 West Pennsylvania Avenue, Urbana, IL 61801, USA;

    Department of Agricultural and Biological Engineering,University of Illinois, 360G AESB, 1304 West Pennsylvania Avenue, Urbana, IL 61801, USA;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    starch hydrolysis; amylose; amylopectin; liquefaction; saccharification; monte carlo simulation;

    机译：淀粉水解;直链淀粉支链淀粉液化;糖化蒙特卡洛模拟;

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