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A study on the dynamics of the zraP gene expression profile and its application to the construction of zinc adsorption bacteria

机译：zraP基因表达谱的动态研究及其在锌吸附细菌构建中的应用

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摘要

Zinc ion plays essential roles in biological chemistry. Bacteria acquire Zn2+ from the environment, and cellular concentration levels are controlled by zinc homeostasis systems. In comparison with other homeo-static systems, the ZraSR two-component system was found to be more efficient in responding to exogenous zinc concentrations. To understand the dynamic response of the bacterium ZraSR two-component system with respect to exogenous zinc concentrations, the genetic circuit of the ZraSR system was integrated with a reporter protein. This study was helpful in the construction of an E. coli system that can display selective metal binding peptides on the surface of the cell in response to exogenous zinc. The engineered bacterial system for monitoring exogenous zinc was successfully employed to detect levels of zinc as low as 0.001 mM, which directly activates the expression of chimeric ompC_t-zinc binding peptide gene to remove zinc by adsorbing a maximum of 163.6 μmol of zinc per gram of dry cell weight. These results indicate that the engineered bacterial strain developed in the present study can sense the specific heavy metal and activates a cell surface display system that acts to remove the metal.
机译：锌离子在生物化学中起着至关重要的作用。细菌从环境中获取Zn2 +，细胞浓度水平由锌稳态系统控制。与其他稳态系统相比，发现ZraSR两组分系统在响应外源锌浓度方面更有效。为了了解细菌ZraSR两组分系统相对于外源锌浓度的动态响应，将ZraSR系统的遗传回路与报告蛋白整合在一起。这项研究有助于构建一个大肠杆菌系统，该系统可以响应外源锌而在细胞表面展示选择性的金属结合肽。通过工程细菌系统监测外源锌已成功用于检测低至0.001 mM的锌水平，该酶直接吸附嵌合的ompC_t-zinc结合肽基因的表达，从而通过吸附每克最多163.6μmol的锌来去除锌。干细胞重量。这些结果表明，在本研究中开发的工程菌菌株可以感知特定的重金属并激活细胞表面展示系统，该系统可以去除金属。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2011年第9期|p.1119-1126|共8页
  • 作者

    Sambandam Ravikumar; Ik-keun Yoo; Sang Yup Lee; Soon Ho Hong;

  • 作者单位

    School of Chemical Engineering and Bioengineering, University of Ulsan, Daehakro 93, Nam-gu, Ulsan 680-749, Republic of Korea;

    School of Chemical Engineering and Bioengineering, University of Ulsan, Daehakro 93, Nam-gu, Ulsan 680-749, Republic of Korea;

    Department of Chemical and Biomolecular Engineering (BK21 Program), Metabolic and Biomolecular Engineering National Research Laboratory, Daejeon, Korea, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Korea, Bioprocess Engineering Research Center, Bioinformatics Research Center, Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Korea;

    School of Chemical Engineering and Bioengineering, University of Ulsan, Daehakro 93, Nam-gu, Ulsan 680-749, Republic of Korea;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    two-component system; zinc; bioadsorption; ompc;

    机译：两部分系统;锌生物吸附ompc;

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