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首页> 外文期刊>Bioprocess and Biosystems Engineering >Proteome-based physiological analysis of the metabolically engineered succinic acid producer Mannheimia succiniciproducens LPK7
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Proteome-based physiological analysis of the metabolically engineered succinic acid producer Mannheimia succiniciproducens LPK7

机译：代谢工程化琥珀酸生产商曼海姆琥珀酸生产蛋白LPK7的基于蛋白质组的生理分析

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摘要

Using two-dimensional gel electrophoresis (2-DE) and mass spectrometry, the proteome of a metabolically engineered succinic acid-overproducing bacterium, Mannheimia succiniciproducens LPK7, was examined and compared with that of its wild type strain, MBEL55E, to elucidate the physiological and metabolic changes responsible for succinic acid overproduction and cell growth. Comparative proteomic studies clearly showed that the expression levels of enzymes involved in the ATP formation and consumption (AtpD, Ppa, SerS, ProS, Pnp, PotD, MalK, RbsB, and TbpA), pyruvate metabolism (AceF and Lpd), glycolysis (GapA, Pgk, Fba, and TpiA), and amino acid biosynthesis (Asd, DapA, DapD, Gdh, ArgD, and ArgG) varied significantly in the LPK7 strain compared with those in the MBEL55E strain. Based on the comparative proteome profiling, the formation of pyruvic acid, a newly formed byproduct in the engineered LPK7 strain, could be reduced by adding into the culture mediumrnpantothenate and L-cysteine, which serve as precursors of CoA biosynthesis.
机译：使用二维凝胶电泳（2-DE）和质谱法，研究了代谢工程改造的琥珀酸过量生产细菌曼海姆氏琥珀酸LPK7的蛋白质组，并将其与野生型菌株MBEL55E进行了比较，从而阐明了引起琥珀酸过量生产和细胞生长的代谢变化。比较蛋白质组学研究清楚地表明，参与ATP形成和消耗的酶的表达水平（AtpD，Ppa，SerS，ProS，Pnp，PotD，MalK，RbsB和TbpA），丙酮酸代谢（AceF和Lpd），糖酵解（GapA）与MBEL55E菌株相比，LPK7菌株的氨基酸，Pgk，Fba和TpiA），氨基酸生物合成（Asd，DapA，DapD，Gdh，ArgD和ArgG）差异显着。根据比较蛋白质组分析，通过向培养基中加入泛酸和L-半胱氨酸作为CoA生物合成的前体，可以减少工程化LPK7菌株中新形成的副产物丙酮酸的形成。

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  • 来源
    《Bioprocess and Biosystems Engineering》 |2010年第1期|97-107|共11页
  • 作者

    Jeong Wook Lee; Sang Yup Lee;

  • 作者单位

    Metabolic and Biomolecular Engineering National Research Laboratory., Department of Chemical and Biomolecular Engineering (BK21 Program), BioProcess Engineering Research Center, KAIST, 335 Gwahangno, Yuseong-gu, Daejeon 305-701, Republic of Korea Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST, 335 Gwahangno, Yuseong-gu, Daejeon 305-701, Republic of Korea;

    Metabolic and Biomolecular Engineering National Research Laboratory., Department of Chemical and Biomolecular Engineering (BK21 Program), BioProcess Engineering Research Center, KAIST, 335 Gwahangno, Yuseong-gu, Daejeon 305-701, Republic of Korea Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST, 335 Gwahangno, Yuseong-gu, Daejeon 305-701, Republic of Korea Department of Bio and Brain Engineering and Bioinformatics Research Center, KAIST, 335 Gwahangno, Yuseong-gu, Daejeon 305-701, Republic of Korea;

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  • 正文语种 eng
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  • 关键词

    mannheimia; proteome; succinic acid; pyruvic acid; CoA;

    机译：曼海姆病蛋白质组琥珀酸丙酮酸CoA;

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